Acetone extraction of mcl-PHA from Pseudomonas putida KT2440
Acetone extraction of mcl-PHA from Pseudomonas putida KT2440
复制标题
DOI：
10.1016/j.mimet.2006.03.015
复制
发表时间：
2006-11-01
影响因子：
2.2
通讯作者：
Ramsay, Bruce A.
中科院分区：
文献类型：
--
作者：
Jiang, Xuan;Ramsay, Juliana A.;Ramsay, Bruce A.
建立了一种从恶臭假单胞菌中提取中长链聚3-羟基烷酸酯（mcl-PHA）的方法。经测定，如果将含有mcl-PHA的干燥恶臭假单胞菌生物质在20体积的甲醇中洗涤5分钟，然后在10体积的丙酮中索氏提取5小时，则几乎所有的PHA都可以回收，而没有可检测到的分子量损失。与含有较少PHA的生物质相比，含有较高量PHA的生物质在预处理步骤期间需要较少的甲醇，但在溶剂萃取步骤中需要较多的丙酮。进一步的纯化可以通过将PHA再溶解在丙酮中并在冷甲醇中再沉淀来实现。241和275 nm处的UV光谱可用作产品纯度的指示。(c)2006 Elsevier B. V.保留所有权利。
A methodology was developed for the extraction of medium-chain-length poly-3-hydroxyalkanoates (mcl-PHA) from Pseudomonas putida. It was determined that if dry P. putida biomass containing mcl-PHA was washed in 20 volumes of methanol for 5 min followed by Soxhlet extraction in 10 volumes of acetone for 5 h, almost all of the PHA could be recovered with no detectable loss of molecular weight. Biomass containing higher amounts of PHA required less methanol during the pretreatment step but more acetone in the solvent extraction step than biomass containing less PHA. Further purification could be achieved by redissolving the PHA in acetone and reprecipitating in cold methanol. UV spectroscopy at 241 and 275 nm could be used as an indication of product purity. (c) 2006 Elsevier B.V. All rights reserved.