Evolutionary dynamics and genomic features of the Elizabethkingia anophelis 2015 to 2016 Wisconsin outbreak strain.
Evolutionary dynamics and genomic features of the Elizabethkingia anophelis 2015 to 2016 Wisconsin outbreak strain.
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DOI：
10.1038/ncomms15483
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发表时间：
2017-05-24
影响因子：
16.6
通讯作者：
Brisse S
中科院分区：
文献类型：
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作者：
Perrin A;Larsonneur E;Nicholson AC;Edwards DJ;Gundlach KM;Whitney AM;Gulvik CA;Bell ME;Rendueles O;Cury J;Hugon P;Clermont D;Enouf V;Loparev V;Juieng P;Monson T;Warshauer D;Elbadawi LI;Walters MS;Crist MB;Noble-Wang J;Borlaug G;Rocha EPC;Criscuolo A;Touchon M;Davis JP;Holt KE;McQuiston JR;Brisse S
威斯康星州发生了一次规模巨大的按蚊感染暴发。在这里，我们表明，它是由一个单一的菌株与13个特征基因组区域。引人注目的是，爆发分离株显示出加速的进化速度和非典型的突变谱。揭示了六个具有独特的时间和地理动态的系统发育亚群，它们的最后一个共同祖先存在于第一次人类感染前大约一年。与其他E.在按蚊中，爆发菌株具有由整合和接合元件插入引起的DNA修复mutY基因破坏。这种基因组变化可能导致了爆发菌株的高进化率，并可能增加了其适应性，因为在爆发期间发生了许多蛋白质编码基因的突变。这一由自然发生的突变细菌病原体引起的爆发的独特发现提供了病原体进化动力学对传染病流行病学的潜在影响的戏剧性例子。 按蚊嗜蚊病是一种新兴的高度耐药性病原体。Perrin及其同事对2015/2016 E.在威斯康星州爆发的按蚊中，发现了大量的遗传多样性，加速了进化速度，并在DNA修复基因mutY中发现了破坏性突变。
An atypically large outbreak of Elizabethkingia anophelis infections occurred in Wisconsin. Here we show that it was caused by a single strain with thirteen characteristic genomic regions. Strikingly, the outbreak isolates show an accelerated evolutionary rate and an atypical mutational spectrum. Six phylogenetic sub-clusters with distinctive temporal and geographic dynamics are revealed, and their last common ancestor existed approximately one year before the first recognized human infection. Unlike other E. anophelis, the outbreak strain had a disrupted DNA repair mutY gene caused by insertion of an integrative and conjugative element. This genomic change probably contributed to the high evolutionary rate of the outbreak strain and may have increased its adaptability, as many mutations in protein-coding genes occurred during the outbreak. This unique discovery of an outbreak caused by a naturally occurring mutator bacterial pathogen provides a dramatic example of the potential impact of pathogen evolutionary dynamics on infectious disease epidemiology. Elizabethkingia anophelis is an emerging pathogen of high antimicrobial resistance. Perrin and colleagues sequenced isolates of a 2015/2016 E. anophelis outbreak in Wisconsin and found substantial genetic diversity, accelerated evolutionary rate and a disruptive mutation in the DNA repair gene mutY.