In vivo gene delivery to the lung using polyethylenimine and fractured polyamidoamine dendrimers
In vivo gene delivery to the lung using polyethylenimine and fractured polyamidoamine dendrimers
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DOI：
10.1002/1521-2254(200007/08)2:4
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发表时间：
2000-07
期刊：
影响因子：
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通讯作者：
C. Rudolph;J. Lausier;S. Naundorf;R. Müller;J. Rosenecker
中科院分区：
文献类型：
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作者：
C. Rudolph;J. Lausier;S. Naundorf;R. Müller;J. Rosenecker
将基因转移到气道中可能对治疗慢性肺部疾病（如囊性纤维化）很重要。在过去的几年中，已经尝试使用不同的病毒和非病毒载体系统有效地将DNA传递到肺部。病毒载体和阳离子脂质已经进行了深入的测试，但阳离子聚合物如聚乙烯亚胺（PEI） 25 kDa和断裂的聚酰胺胺树状大分子将DNA传递到气道的性能尚未研究。表面活性剂制剂已被证明影响肺腺病毒和裸质粒DNA介导的体内基因转移。我们研究了支化的PEI 25kda和断裂的树枝状大分子在小鼠肺中的基因传递效率，并研究了表面活性剂对PEI 25kda介导的基因向肺转移的影响。
Gene transfer into the airways could be of importance for the treatment of chronic lung diseases such as cystic fibrosis. In the past few years several attempts have been made to effectively deliver DNA to the lung using different viral and non‐viral vector systems. Viral vectors and cationic lipids have been tested intensively but the properties of cationic polymers such as polyethylenimine (PEI) 25 kDa and fractured polyamidoamine dendrimers to deliver DNA to the airways have not been studied. Surfactant preparations have been shown to influence pulmonary adenoviral and naked plasmid DNA mediated gene transfer in vivo. We investigated the gene delivery efficiency of branched PEI 25 kDa and fractured dendrimers to the murine lung in vivo and also examined the effect of surfactant on PEI 25 kDa mediated gene transfer to the lung.