Near-Infrared Light-Triggered Photodynamic Therapy and Apoptosis Using Upconversion Nanoparticles With Dual Photosensitizers
Near-Infrared Light-Triggered Photodynamic Therapy and Apoptosis Using Upconversion Nanoparticles With Dual Photosensitizers
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DOI：
10.3389/fbioe.2020.00275
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发表时间：
2020-04-16
影响因子：
5.7
通讯作者：
Park, Yong Il
中科院分区：
文献类型：
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作者：
Lee, Song Yeul;Lee, Ruda;Park, Yong Il
上转换纳米粒子（UCNPs）可以被近红外（NIR）光激发的解释是光动力学治疗（PDT）领域的一个有趣的话题。然而，常规UCNP的PDT效率由于980 nm光源的低量子产率和过热效应而受到限制。在这项研究中，使用波长为808 nm的光源作为Nd掺杂的UCNPs的激发源，以解决过热效应。合成了具有核@壳结构的UCNPs（NaYF 4：Yb，Er，Nd@NaYF4：Yb，Nd）以提高上转换发光效率。使用双色发射的掺铒UCNPs和双光敏剂（二氢卟酚e6和孟加拉玫瑰红）来增强PDT。每种光敏剂都可以吸收UCNP的红光和绿色光，分别产生活性氧（ROS）。双光敏剂体系中的ROS生成量显著高于单光敏剂体系。此外，PDT诱导免疫原性细胞凋亡。在这项研究中，通过利用一个高效的PDT剂，PDT诱导的细胞凋亡进行了研究的生物标志物分析。
Elucidation of upconversion nanoparticles (UCNPs) that can be excited by near-infrared (NIR) light is an interesting topic in the field of photodynamic therapy (PDT). However, the PDT efficiency of conventional UCNPs is limited due to the low quantum yield and overheating effect of the 980 nm light source. In this study, a light source with a wavelength of 808 nm was used as an excitation source for Nd-doped UCNPs to solve the overheating effect. UCNPs with a core@shell structure (NaYF4:Yb,Er,Nd@NaYF4:Yb,Nd) were synthesized to increase the upconversion emission efficiency. Dual-color emitting Er-doped UCNPs and dual photosensitizers (Chlorin e6 and Rose Bengal) were used for enhanced PDT. Each photosensitizer could absorb red and green emissions of the UCNPs to generate reactive oxygen species (ROS), respectively. The ROS generation in a dual photosensitizer system is significantly higher than that in a single photosensitizer system. Additionally, PDT induces immunogenic apoptosis. In this study, by utilizing a highly efficient PDT agent, PDT-induced apoptosis was studied by biomarker analysis.