Effects of extracellular DNA from Candida albicans and pneumonia-related pathogens on Candida biofilm formation and hyphal transformation
Effects of extracellular DNA from Candida albicans and pneumonia-related pathogens on Candida biofilm formation and hyphal transformation
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DOI：
10.1111/jam.12483
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发表时间：
2014-06-01
影响因子：
4
通讯作者：
Miyake, Y.
中科院分区：
文献类型：
--
作者：
Sapaar, B.;Nur, A.;Miyake, Y.
目的：本研究的目的是研究从白色念珠菌和肺炎相关病原体、铜绿假单胞菌和金黄色葡萄球菌中纯化的基因组 DNA 对 3 种念珠菌（白色念珠菌、光滑念珠菌和热带念珠菌）体外生物膜形成和形态变化的影响。方法和结果：通过结晶紫测定和菌落形成单位评估生物膜形成很重要。通过扫描电子显微镜和荧光显微镜评估生物膜的形态特征。添加低浓度 DNA（
Aims: The aim of this study was to investigate the effects of genomic DNA purified from Candida albicans and pneumonia-related pathogens, Pseudomonas aeruginosa and Staphylococcus aureus, on in vitro biofilm formation and morphological change of 3 Candida species (C. albicans, C. glabrata, and C. tropicalis).Methods and Results: Biofilm formation was evaluated by the crystal violet assay and colony-forming unit counts. Morphological characteristics of biofilms were evaluated by scanning electron microscopy and fluorescence microscopy. Addition of DNA at a low concentration (