Proteomics analysis of the actions of grape seed extract in rat brain: technological and biological implications for the study of the actions of psychoactive compounds.
Proteomics analysis of the actions of grape seed extract in rat brain: technological and biological implications for the study of the actions of psychoactive compounds.
复制标题
葡萄籽提取物在大鼠大脑中作用的蛋白质组学分析：对精神活性化合物作用研究的技术和生物学意义。
DOI：
10.1016/j.lfs.2005.12.008
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发表时间：
2006
期刊：
影响因子：
6.1
通讯作者：
Meleth,Sreelatha
中科院分区：
文献类型：
--
作者：
Kim,Helen;Deshane,Jessy;Barnes,Stephen;Meleth,Sreelatha
葡萄籽提取物(GSE)是一种常见的膳食补充剂，其抗氧化活性归因于其原花青素(单体多酚的低聚物)的含量。从蓝莓和大豆中提取的类似的富含多酚的制剂已经显示出对卵巢切除引起的或与年龄相关的认知障碍的保护作用，这表明这些多酚制剂引起的分子变化与行为益处有关。我们假设，摄入富含多酚的制剂，如GSE，将表现为蛋白质变化，这与神经保护是一致的。蛋白质组学技术，即2D凝胶电泳和质谱学，确定了成年大鼠摄入GSE粉末制剂后，大脑中诱导的特定蛋白质的定量变化。正如最近报告的那样，[DeShane，J.，Chaves，L.，Sarikonda，K.V.，Isbell，S.，Wilson，L.，Kirk，M.，GRubbs，C.，Barnes，S.，Meleth，S.和Kim，H.，2004。葡萄籽提取物对大鼠脑蛋白质调控的蛋白质组学分析。农业和食品化学杂志52,7872-7883。]，大多数受影响蛋白质的变化方向与阿尔茨海默病或痴呆转基因小鼠模型中的蛋白质变化方向相反。保守的结论是，GSE通过以特定的方式影响特定的蛋白质，具有神经保护活性。在这一章中，讨论了蛋白质组学类型分析的元素，这些元素展示了技术的力量，能够发现参与大脑对刺激的反应的蛋白质，无论是膳食补充剂还是精神活性药物。此外，GSE影响与认知障碍有关的蛋白质的事实表明，GSE可能通过维持神经系统的整体生存能力对精神活性药物的作用产生影响。
Grape seed extract (GSE) is a commonly available dietary supplement taken for the anti-oxidant activity that's attributed to its proanthocyanidin (oligomers of monomeric polyphenols) content. Similar polyphenol-enriched preparations from blueberries and soy have shown protection against ovariectomy-induced or age-related cognitive deficits, suggesting that the molecular changes induced by these polyphenol preparations correlated with behavioral benefit. We hypothesized that ingestion of polyphenol-enriched preparations such as GSE would be manifested as protein changes that would be consistent with neuroprotection. Proteomics technology, namely 2D gel electrophoresis and mass spectrometry, identified quantitative changes in specific proteins induced in adult rat brain following ingestion of a powdered preparation of GSE. As recently reported [Deshane, J., Chaves, L., Sarikonda, K.V., Isbell, S., Wilson, L., Kirk, M., Grubbs, C., Barnes, S., Meleth, S. and Kim, H., 2004. Proteomics analysis of rat brain protein modulations by grape seed extract. Journal of Agricultural and Food Chemistry 52, 7872-7883.], the direction of change for the majority of the affected proteins was opposite to the direction the proteins were changed in either Alzheimer disease or in transgenic mouse models of dementia. A conservative conclusion is that GSE has neuroprotective activity, by affecting specific proteins in particular ways. In this chapter, elements of proteomics-type analysis are discussed that demonstrate the power of the technology to enable discovery of proteins involved in the response of the brain to a stimulus whether it be a dietary supplement, or a psychoactive drug. The fact that GSE affects proteins implicated in cognitive disorders suggests moreover that GSE may have impact on the actions of psychoactive drugs by maintaining an overall viability of the nervous system.