Platelet-derived growth factor promotes proliferation of erythropoietic progenitor cells in vitro.
Platelet-derived growth factor promotes proliferation of erythropoietic progenitor cells in vitro.
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血小板源性生长因子在体外促进红细胞生成祖细胞的增殖。
DOI：
10.1172/jci110869
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Kulkarni,V
中科院分区：
文献类型：
--
作者：
Dainiak,N;Davies,G;Kalmanti,M;Lawler,J;Kulkarni,V
为了研究体外最佳红细胞生成的血清需求，我们研究了培养中红系祖细胞增殖对血小板衍生生长因子（PDGF）的反应。与人全血血清或胎牛血清培养的骨髓细胞相比，用贫血小板血浆衍生血清（PDS）培养的骨髓细胞形成的红系集落较少（P <0.05）。用凝血酶处理洗涤过的血小板会释放出一种低分子量（小于100，000）因子，这种因子可促进集落生长。这种分泌的因子似乎是PDGF，这是基于部分纯化的和免疫学纯的PDGF将含PDS的培养物的集落形成能力恢复到在具有全血血清或胎牛血清的对照培养物中发现的水平的70-96%的能力。血小板源生长因子的集落生长的增强，注意到只有在骨髓培养补充促红细胞生成素和PDS。通过对高输血、红细胞增多小鼠和禁食大鼠的测定，排除了PDGF制剂中存在生物活性促红细胞生成素。尽管PDGF在含有最佳浓度的促红细胞爆发活性（BPA）的骨髓培养物中刺激红细胞爆发形成，但它不影响循环红细胞爆发的增殖，不管加入培养物中的BPA浓度如何。我们的结论是，血小板衍生生长因子是骨髓培养中最佳红系祖细胞增殖的血清决定因素。PDGF的活性不同于表观红细胞特异性生长因子促红细胞生成素和BPA的活性。
To investigate serum requirements for optimal erythropoiesis in vitro, we studied the response of erythroid progenitor cell proliferation in culture to platelet-derived growth factor (PDGF). Human bone marrow cells cultured with platelet-poor plasma-derived serum (PDS) form fewer erythroid colonies than do cells cultured with human whole blood serum or fetal calf serum (P less than 0.05). Treatment of washed platelets with thrombin releases a low molecular weight (less than 100,000) factor that enhances colony growth. This secreted factor appears to be PDGF, based upon the ability of partially purified and electrophoretically pure PDGF to restore colony-forming capacity of PDS-containing cultures to 70-96% of the level found in control cultures with whole blood serum or fetal calf serum. Enhancement of colony growth by PDGF was noted only in marrow cultures supplemented with erythropoietin and PDS. Presence of bioactive erythropoietin in PDGF preparations was excluded by assay in hypertransfused, polycythemic mice, and in fasted rats. Although PDGF stimulates erythroid burst formation in marrow cultures containing optimal concentrations of burst-promoting activity (BPA), it does not influence proliferation of circulating erythroid bursts, regardless of BPA concentration added to culture. We conclude that PDGF is a serum determinant of optimal erythroid progenitor cell proliferation in marrow culture. The activity of PDGF is distinct from that of the apparent erythroid specific growth factors erythropoietin and BPA.