Basic fibroblast growth factor(FGF-2) in mouse tooth morphogenesis
Basic fibroblast growth factor(FGF-2) in mouse tooth morphogenesis
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DOI：
10.3109/08977199809117188
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发表时间：
1998-01-01
期刊：
影响因子：
1.8
通讯作者：
Maharajan, V
中科院分区：
文献类型：
--
作者：
Russo, LG;Maharajan, P;Maharajan, V
本研究采用免疫组织化学方法观察了碱性成纤维细胞生长因子（FGF-2）在小鼠牙胚发育过程中的时空表达。用抗FGF-2多克隆抗体孵育含有不同发育阶段牙齿的小鼠胚胎头部（妊娠13-18天）的旁组织切片，并用链霉亲和素-生物素复合物-HRP系统和AEC染色证实阳性结合。在牙板阶段，我们没有观察到FGF-2染色。在芽期，可见轻微染色，仅限于一些上皮细胞。染色强度在帽状期增加。钟状期，星网状细胞染色强烈。随后，成牙本质细胞和牙本质基质染色较深，但上皮细胞染色较淡。牙本质和牙乳头的细胞外基质染色非常强烈，而釉质基质呈阴性。这些结果表明FGF-2参与牙齿形成细胞的分化而不是增殖。特别是，FGF-2似乎参与成牙本质细胞分化和牙本质基质沉积。FGF-2在小鼠牙齿发育中的时空特异性分布模式强调了FGF-2在哺乳动物牙齿发育中的重要性。
This study describes the spatio-temporal expression of basic Fibroblast growth factor (FGF-2) during odontogenesis of mouse as revealed by immunohistology. Parasagittal sections of mouse embryo head (13-18 day of gestation) containing various stages of developing tooth-were incubated with a polyclonal anti-FGF-2 antibody and positive binding was evidentiated by using Streptavidin-Biotin complex-HRP system and AEC staining. We observed no FGF-2 staining at the dental lamina stage. At the bud stage slight staining is seen, limited to some epithelial cells. The intensity of the staining increases at the cap stage. In the bell stage, the stellate reticulum cells stain intensely. Later, odontoblasts and the dentin matrix stain deeply; but the epithelial cells stain faint. The extra cellular matrix of the dentin and dental papilla stain very intense but the enamel matrix is found negative. These results indicate the participation of FGF-2 in differentiation rather than in proliferation of tooth-forming cells. In particular, it appears that FGF-2 participates in odontoblast differentiation and in dentin matrix deposition. The spatio-temporally specific distribution pattern of FGF-2 in developing mouse tooth reported here emphasizes the importance of FGF-2 in mammalian odontogenesis.