N-Isopropylacrylamide-Modified Polyethylenimines as Effective Gene Carriers
N-Isopropylacrylamide-Modified Polyethylenimines as Effective Gene Carriers
复制标题
N-异丙基丙烯酰胺修饰的聚乙烯亚胺作为有效的基因载体
DOI：
10.1002/mabi.201200249
复制
发表时间：
2012-12-01
影响因子：
4.6
通讯作者：
Chen, Xuesi
中科院分区：
文献类型：
--
作者：
Tian, Huayu;Li, Feifan;Chen, Xuesi
25?kDa branched polyethylenimines are modified by N-isopropylacrylamide via Michael addition. An agarose gel retardation assay shows that all derivatives display good binding affinity toward plasmid DNA. The modified PEI-25K shows lower cytotoxicity in MTT assay and better transfection efficiency than unmodified PEI-25K in HeLa cells. The endocytosis efficiency of the optimized complexes is determined to be 99.9% by flow cytometry. More interestingly, although the derivatives are not designed to conjugate with targeting ligands or nuclear localization signals, confocal laser scanning microscopy (CLSM) demonstrates that the optimized derivative results in increased endocytosis and strongly enhanced nuclear uptake compared with PEI-25K.
