Enzymatic Fuel Cell-Based Self-Powered Homogeneous Immunosensing Platform via Target-Induced Glucose Release: An Appealing Alternative Strategy for Turn-On Melamine Assay
Enzymatic Fuel Cell-Based Self-Powered Homogeneous Immunosensing Platform via Target-Induced Glucose Release: An Appealing Alternative Strategy for Turn-On Melamine Assay
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基于酶燃料电池的自供电均质免疫传感平台，通过目标诱导葡萄糖释放：开启三聚氰胺测定的一种有吸引力的替代策略
DOI：
10.1021/acsami.7b07104
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发表时间：
2017-10-18
影响因子：
9.5
通讯作者：
Li, Feng
中科院分区：
文献类型：
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作者：
Gu, Chengcheng;Gai, Panpan;Li, Feng
基于酶燃料电池（EFC）的自供电生物传感器由于其在整个检测过程中不需要额外的电源，具有简单、快速、成本低、抗干扰、使用方便等优点而受到广泛关注。在此，我们首次提出了一种基于EFC的自供电均相免疫传感平台，将靶诱导生物燃料释放和生物偶联免疫测定相结合，用于超灵敏的三聚氰胺（ME）检测。在该设计中，生物燃料，即，葡萄糖分子，被捕获在带正电荷的介孔二氧化硅纳米颗粒的孔中，并被修饰的AuNP标记的抗ME抗体覆盖（AuNPs-Ab），目标ME的存在触发了由于经由免疫反应去除葡萄糖酸而导致的捕获的葡萄糖释放，这导致在生物阳极处由葡萄糖氧化产生的电子转移到生物阴极，并且因此，自供电免疫传感器的开路电压显著提高，实现了对ME的超灵敏开启检测，ME检测的最低检测限为2.1 pM（S/N = 3），上级优于文献报道的检测限。值得注意的是，真实的牛奶样品不需要特殊的样品预处理来检测ME，因为基于EFC的自供电生物传感器的良好抗干扰能力和均相免疫测定的优异选择性。因此，这种吸引人的自供电均相免疫传感平台作为食品安全领域中便携式和现场生物测定的成功原型具有很大的希望。
Enzymatic fuel cell (EFC)-based self-powered biosensors have attracted considerable attention because of their unique feature of no need for extra power sources during the entire detection process, which endows them with the merits of simplicity; rapidness, low cost, anti -interference, and ease of use. Herein, we proposed, for the first time, an EFC-based self-powered homogeneous immunosensing platform by integrating the target-induced biofuel release and bioconjugate immunoassay for ultrasensitive melamine (ME) detection. In this design, the biofuel, i.e., glucose molecules, was entrapped in the pores of positively charged mesoporous silica nanoparticles and, capped by the biogate AuNPs-labeled anti -ME antibody (AuNPs-Ab), The presence of the target ME triggered the entrapped glucose release due to the removal of the biogate via imm-uhoreaction, which resulted in the transfer of electrons produced by glucose oxidation at the bioanode to the biocathode, and thus, the open-circuit voltage of the EFC-based self powered immunosensor dramatically increased, realizing the ultrasensitive turn-on assay for ME. The limit of detection for ME assay was down to 2.1 pM (S/N = 3), superior to those previously reported in the literature. Notably, real milk samples need no special sample pretreatment for the detection of ME because of the good anti -interference ability of EFC-based self-powered biosensors and the excellent selectivity of the homogeneous immunoassay. Therefore, this, appealing self-powered homogeneous immunosensing platform holds great promise as a successful prototype of portable and on-site bioassay in the field of food safety.