INFECTION OF HTLV-III/LAV IN HTLV-I-CARRYING CELLS MT-2 AND MT-4 AND APPLICATION IN A PLAQUE-ASSAY
INFECTION OF HTLV-III/LAV IN HTLV-I-CARRYING CELLS MT-2 AND MT-4 AND APPLICATION IN A PLAQUE-ASSAY
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DOI：
10.1126/science.2992081
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发表时间：
1985-01-01
期刊：
影响因子：
56.9
通讯作者：
YAMAMOTO, N
中科院分区：
文献类型：
--
作者：
HARADA, S;KOYANAGI, Y;YAMAMOTO, N
人T细胞系MT-2和MT-4携带人T细胞白血病病毒I型（HTLV-1）。当MT-2和MT-4感染HTLV-III，获得性免疫缺陷综合征（AIDS）的可能病原体，观察到快速的细胞病变效应和细胞毒性，这使得有可能在空斑形成试验中滴定生物活性病毒。细胞病变的影响之前，快速诱导和增加HTLV-III抗原所揭示的免疫荧光和免疫沉淀。HTLV-III的活动被中和的人抗病毒的抗体时，使用免疫荧光和空斑试验。用淋巴结病相关病毒（LAV 1）获得了基本相同的结果。
The human T-cell lines MT-2 and MT-4 carry the human T cell leukemia virus type I (HTLV-1). When MT-2 and MT-4 were infected with HTLV-III, the probably etiologic agent of the acquired immune deficiency syndrome (AIDS), rapid cytopathogenic effects and cytotoxicity were observed that made it possible to titrate the biologically active virus in a plaque-forming assay. The cytopathogenic effects were preceded by the rapid induction and increase of HTLV-III antigens as revealed by immunofluorescence and immunoprecipitation. Activities of HTLV-III were neutralized by the human antibodies against the virus when immunofluorescence and plaque assays were used. Essentially the same results were obtained with the lymphadenopathy-associated virus (LAV1).