Measurement of Cortisol and Testosterone in Hair of Obese and Non-Obese Human Subjects
Measurement of Cortisol and Testosterone in Hair of Obese and Non-Obese Human Subjects
复制标题
DOI：
10.1055/s-0034-1374609
复制
发表时间：
2014-06-01
影响因子：
1.8
通讯作者：
Van Uum, S.
中科院分区：
文献类型：
--
作者：
Chan, J.;Sauve, B.;Van Uum, S.
目的：毛发分析已被证明可以准确反映药物滥用、环境毒素和外源性激素的暴露情况。我们测试了测量健康和肥胖受试者头发中皮质醇和睾酮的可行性。测量方法：使用最初为唾液开发的改良免疫测定法（ELISA）。从年轻的非肥胖和肥胖患者中收集头发、尿液和血液样本。结果：非肥胖组和肥胖组的压力感知（PSS）无显著性差异。头发皮质醇水平与体重（r = 0.27，p < 0.05）和收缩压（r = 0.28，p < 0.05）显著相关，而与BMI的相关性未达到统计学意义（p = 0.063）。头发皮质醇水平与年龄或尿皮质醇无关。发睾酮与年龄（r =-0.47，p < 0.05）和BMI（r =-0.40，p < 0.05）呈负相关。毛发睾酮与游离雄激素指数（FAI）之间的相关性未达到统计学显著性（p = 0.098）。发皮质醇/发睾酮比值（C/T）肥胖组高于青年非肥胖组。C/T比值与年龄（r = 0.56，p < 0.01）、腰围（r = 0.63，p < 0.01）、BMI（r = 0.62，p < 0.01）呈正相关，与FAI相关性不显著。头发C/T比值与年龄、BMI和腰围的相关性优于单独的头发皮质醇或睾酮。由于毛发收集是非侵入性的，并且不受时刻变化的影响，因此毛发中激素的测量是研究和可能的临床实践中的有用工具。
Objective: Hair analysis has been demonstrated to accurately reflect exposure to drug abuse, environmental toxins and exogenous hormones. We tested the feasibility of measuring cortisol and testosterone in hair of healthy and obese subjects.Measurements: A modified immunoassay (ELISA) originally developed for saliva was used. Hair, urine and blood samples were collected from young non-obese and obese patients. Perceived stress (PSS) was measured using a validated questionnaire.Results: There was no difference in PSS between non-obese and obese subjects. Hair cortisol levels were significantly correlated with weight (r = 0.27, p < 0.05) and systolic blood pressure (r = 0.28, p < 0.05), while the correlation with BMI did not reach statistical significance (p = 0.063). Hair cortisol levels did not correlate with age or urinary cortisol. There was a negative correlation between hair testosterone and age r = -0.47, p < 0.05) and BMI (r = -0.40, p < 0.05). The correlation between hair testosterone and free androgen index (FAI) did not reach statistical significance (p = 0.098). The ratio of hair cortisol over hair testosterone (C/T) was higher in the obese group than in the young non-obese group. The C/T ratio correlated positively with age (r = 0.56, p < 0.01), waist circumference (r = 0.63, p < 0.01) and BMI (r = 0.62, p < 0.01), while the correlation between C/T ratio and FAI did not reach statistical significance.Conclusion: Hair cortisol levels increase, while hair testosterone levels decrease with obesity. The hair C/T ratio was significantly correlated with age, BMI and waist circumference better than hair cortisol or testosterone alone. As hair collection is non-invasive and is not influenced by moment-to-moment variations, the measurement of hormones in hair is a useful tool in research and possibly clinical practice.