Comprehensive analysis of circular RNA profiles in skeletal muscles of aging mice and after aerobic exercise intervention
Comprehensive analysis of circular RNA profiles in skeletal muscles of aging mice and after aerobic exercise intervention
复制标题
衰老小鼠骨骼肌和有氧运动干预后的环状RNA谱的综合分析。
DOI：
10.18632/aging.102932
复制
发表时间：
2020-03-31
期刊：
影响因子：
5.2
通讯作者：
Xu, Lingyan
中科院分区：
文献类型：
--
作者：
Guo, Mingwei;Qiu, Jin;Xu, Lingyan
衰老诱导细胞和组织中损伤的逐渐积累，这导致生理功能障碍。增龄性肌肉功能障碍在老年人群中较为常见，严重影响了老年人的身体活动和生活质量，有氧训练可对抗或缓解这一现象。环状RNA（circRNA）在各种生理过程中发挥重要作用，但它们在衰老相关的肌肉功能障碍中的作用还不清楚。在这项研究中，我们使用RNA测序对久坐的年轻和老龄小鼠以及有氧运动的老龄小鼠的四头肌中的circRNA谱进行了全面分析。我们的研究结果确定了衰老和有氧运动因素改变的circRNA。然后通过基因本体富集分析对它们的宿主基因进行预测和分析。重要的是，我们发现，与年轻小鼠相比，circBBS 9在衰老中的水平降低，而在运动与久坐的衰老小鼠中的表达升高。此外，我们还对circBBS 9靶基因进行了GO和KEGG分析，并建立了circBBS 9-miRNA-mRNA相互作用网络。我们的研究结果表明，circBBS 9可能通过介导有氧训练干预的益处在肌肉衰老中发挥积极作用，因此可能成为对抗衰老相关肌肉功能障碍的新治疗靶点。
Aging induces gradual accumulation of damages in cells and tissues, which leads to physiological dysfunctions. Aging-associated muscle dysfunction is commonly seen in aged population and severely affects their physical activity and life quality, against which aerobic training has been shown to exert antagonizing or alleviating effects. Circular RNAs (circRNAs) play important roles in various physiological processes, yet their involvement in aging-associated muscle dysfunction is not well understood. In this study, we performed comprehensive analysis of circRNAs profiles in quadriceps muscles in sedentary young and aging mice, as well as aging mice with aerobic exercise using RNA sequencing. Our results identified circRNAs altered by factors of aging and aerobic exercise. Their host genes were then predicted and analyzed by gene ontology enrichment analysis. Importantly, we found that circBBS9 featured decreased levels in aging compared to young mice and elevated expression in exercise versus sedentary aging mice. Besides, we performed GO and KEGG analysis on circBBS9 target genes, as well as established the circBBS9-miRNA- mRNAs interaction network. Our results indicate that circBBS9 may play active roles in muscle aging by mediating the benefits of aerobic training intervention, thus may serve as a novel therapeutic target combating aging-associated muscle dysfunction.