Gap junction and purinergic p2 receptor proteins as a functional unit: Insights from transcriptomics
Gap junction and purinergic p2 receptor proteins as a functional unit: Insights from transcriptomics
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DOI：
10.1007/s00232-007-9039-7
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发表时间：
2007-06-01
影响因子：
2.4
通讯作者：
Scemes, Eliana
中科院分区：
文献类型：
--
作者：
Iacobas, Dumitru A.;Suadicani, Sylvia O.;Scemes, Eliana
间隙连接和嘌呤能P2受体（P2Rs）可被视为属于一个共同的功能单元，因为它们都参与细胞间钙信号的传递。我们之前已经证明，Gja1基因的缺失会改变许多编码具有不同功能的蛋白质的基因的表达水平，包括嘌呤能受体（P2Rs），并发现在野生型组织中协同或拮抗表达的基因更容易在cx43 -null中受到类似或相反的调控。我们现在已经探索了使用基因表达的协调分析作为一种策略来识别编码功能相关蛋白的相互关联基因和评估它们的相互联系的下拉。我们的研究结果表明，在大脑和培养的星形胶质细胞中，这些共表达基因中的一些编码蛋白质是P2R信号转导途径的组成部分，并/或直接与这些受体相互作用，包括间隙连接蛋白connexin43 （Cx43）和Cx45以及泛连接蛋白。本文提出，基因表达的配位分析可能为鉴定属于超分子复合物的蛋白质提供一种新的无偏策略。
Gap junctions and purinergic P2 receptors (P2Rs) can be regarded as belonging to a common functional unit, given that they are involved in the transmission of calcium signals between cells. We have previously shown that deletion of the Gja1 gene alters expression levels of numerous genes encoding proteins with diverse functions, including purinergic receptors (P2Rs), and have found that genes synergistically or antagonistically expressed in wild-type tissues are more prone to be similarly or oppositely regulated in Cx43-nulls. We have now explored the use of coordination analysis of gene expression as a strategy to identify interlinked genes encoding functionally related proteins and pull-downs to evaluate their interlinkage. Our findings indicate that, in brain and in cultured astrocytes, several of these coexpressed genes encode proteins that are components of P2R signal-transduction pathways and/or directly interact with these receptors, including the gap junction protein connexin43 (Cx43) and Cx45 as well as pannexins. It is proposed that coordination analysis of gene expression may provide a novel unbiased strategy for the identification of proteins belonging to supramolecular complexes.