Identification and localization of a Rickettsia sp in Bemisia tabaci (Homoptera: Aleyrodidae)
Identification and localization of a Rickettsia sp in Bemisia tabaci (Homoptera: Aleyrodidae)
复制标题
DOI：
10.1128/aem.72.5.3646-3652.2006
复制
发表时间：
2006-05-01
影响因子：
4.4
通讯作者：
Zchori-Fein, Einat
中科院分区：
文献类型：
--
作者：
Gottlieb, Yuval;Ghanim, Murad;Zchori-Fein, Einat
粉虱（同翅目：粉虱科（Aleyrodidae）是一种吸食树液的昆虫，体内有一种专性共生细菌，被安置在一个叫做细菌组的特殊器官中。这些昆虫也是多种兼性微生物群落的家园，所述兼性微生物群落可包括Hamiltonella、Arsenophonus、Fritchea、Wolbachia和Cardinium spp。本研究利用分子指纹技术对甘薯烟粉虱B生物型的细菌进行了鉴定，并首次在该昆虫科中发现了立克次体。应用PCR和荧光原位杂交技术研究了立克次体的分布、传播和定位。20例以色列B型均检出立克次体。烟粉虱种群筛选，但不是在每个种群内的所有个体。对B.烟粉虱卵、成虫和成虫在肠道和滤泡细胞周围显示出独特的立克次体浓度，以及在血淋巴中的随机分布。我们假设，立克次体进入卵母细胞与细菌细胞，离开这些共生体住房细胞时，鸡蛋奠定，繁殖和传播整个鸡蛋在胚胎发育过程中，随后，分散在整个身体的孵化若虫，不包括细菌。尽管立克次体在粉虱生物学中所起的作用目前尚不清楚，但一方面垂直传播和另一方面部分群体内感染表明，这种表型在某些条件下是有利的，但可能有害到足以阻止固定在其他条件下。
Whiteflies (Homoptera: Aleyrodidae) are sap-sucking insects that harbor "Candidatus Portiera aleyrodidarum," an obligatory symbiotic bacterium which is housed in a special organ called the bacteriome. These insects are also home for a diverse facultative microbial community which may include Hamiltonella, Arsenophonus, Fritchea, Wolbachia, and Cardinium spp. In this study, the bacteria associated with a B biotype of the sweet potato whitefly Bemisia tabaci were characterized using molecular fingerprinting techniques, and a Rickettsia sp. was detected for the first time in this insect family. Rickettsia sp. distribution, transmission and localization were studied using PCR and fluorescence in situ hybridizations (FISH). Rickettsia was found in all 20 Israeli B. tabaci populations screened but not in all individuals within each population. A FISH analysis of B. tabaci eggs, nymphs, and adults revealed a unique concentration of Rickettsia around the gut and follicle cells, as well as a random distribution in the hemolymph. We postulate that the Rickettsia enters the oocyte together with the bacteriocytes, leaves these symbiont-housing cells when the egg is laid, multiplies and spreads throughout the egg during embryogenesis and, subsequently, disperses throughout the body of the hatching nymph, excluding the bacteriomes. Although the role Rickettsia plays in the biology of the whitefly is currently unknown, the vertical transmission on the one hand and the partial within-population infection on the other suggest a phenotype that is advantageous under certain conditions but may be deleterious enough to prevent fixation under others.