LUCIFER YELLOW STAINS DISPLACED AMACRINE CELLS OF THE CHICKEN RETINA DURING EMBRYONIC-DEVELOPMENT

LUCIFER YELLOW STAINS DISPLACED AMACRINE CELLS OF THE CHICKEN RETINA DURING EMBRYONIC-DEVELOPMENT
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DOI:
10.1016/0304-3940(82)90099-4
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发表时间:
1982-01-01
影响因子:
2.5
通讯作者:
VOLLMER, G
VOLLMER, G
中科院分区:
医学4区
文献类型:
--
作者:
LAYER, PG;VOLLMER, G

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用荧光黄[LY]对鸡视网膜胚胎发育过程中节细胞层(GCL)内移位的无长突细胞进行组织学示踪。在染料中孵育整个眼睛导致所有移位的无长突细胞的明亮染色,而神经节细胞和神经胶质细胞不染色。内核层(INL)内部的细胞亚群也被染色。红藻氨酸,这是已知的干扰和杀死无长突细胞系统,完全阻断这些细胞的染色。除了组织学证据,这证实了在GCL中的LY染色细胞是移位的无长突细胞。在GCL中的细胞中,23%(. ±. 3%)为移位无长突型。这些细胞的细胞结构在发育过程中发生了显著变化。
Lucifer Yellow [LY] was used for histological tracing of displaced amacrine cells within the ganglion cell layer (GCL) during the embryonic development of the chicken retina. Incubating whole eyes in the dye leads to bright staining of all displaced amacrine cells, whereas ganglion cells and glial cells are not stained. A subpopulation of cells of the inner part of the inner nuclear layer (INL) are also stained. Kainic acid, which is known to interfere with and kill amacrine cell systems, blocks the staining of these cells fully. This in addition to histological evidence confirms that the LY-stained cells in the GCL are displaced amacrine cells. Of the cells in the GCL, 23% (.+-. 3%) are of the displaced amacrine type. The cytoarchitectural arrangement of these cells changes significantly during development.