ASSAYS FOR 8-HYDROXY-2'-DEOXYGUANOSINE - A BIOMARKER OF INVIVO OXIDATIVE DNA DAMAGE
ASSAYS FOR 8-HYDROXY-2'-DEOXYGUANOSINE - A BIOMARKER OF INVIVO OXIDATIVE DNA DAMAGE
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DOI：
10.1016/0891-5849(91)90078-h
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发表时间：
1991-01-01
影响因子：
7.4
通讯作者：
AMES, BN
中科院分区：
文献类型：
--
作者：
SHIGENAGA, MK;AMES, BN
8-羟基-2′-脱氧鸟苷（oh8dG）是一种高灵敏度和选择性的检测方法，是DNA中鸟嘌呤残基受到羟基自由基攻击而形成的DNA氧化损伤的生物标志物。我们建议，尿中oh8dG的无创测量可用于估计体内氧化损伤。将该测定法应用于不同年龄和不同物种的大鼠尿液样本，提供了该测定法实用性的实例。测量DNA中oh8dG的稳态水平，结合oh8dG和oh8Gua的尿排泄率，为估计DNA氧化损伤及其修复提供了一种强有力的方法。这种方法将有助于研究DNA损伤中氧化应激的关系以及这种损伤在衰老和癌症中的作用。
HPLC with electrochemical detection (HPLC-EC) is a highly sensitive and a selective method for detecting 8-hydroxy-2'-deoxyguanosine (oh8dG), a biomarker of oxidative DNA damage that is formed from hydroxyl radical attack of guanine residues in DNA. We propose that the noninvasive measurement of oh8dG in urine can be used to estimate in vivo oxidative damage. Application of this assay to urine samples obtained from rats of different ages and various species provide examples of the utility of this assay. The measurement of steady-state levels of oh8dG in DNA combined with the urinary excretion rates of oh8dG and oh8Gua, offer a powerful approach for estimating oxidative DNA damage and its repair. This method will be useful for studies designed to investigate the relationship of oxidative stress in DNA damage and the role of this damage in aging and cancer.