The neuroprotective role of morroniside against spinal cord injury in female rats
The neuroprotective role of morroniside against spinal cord injury in female rats
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莫诺苷对雌性大鼠脊髓损伤的神经保护作用
DOI：
10.1016/j.neuint.2021.105105
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发表时间：
2021-07-02
影响因子：
4.2
通讯作者：
Hu, Jian-Guo
中科院分区：
文献类型：
--
作者：
Duan, Fei-Xiang;Shi, Yu-Jiao;Hu, Jian-Guo
脊髓损伤（SCI）是一种致残性疾病，如果没有有效的治疗，通常会导致永久性神经功能缺损。氧化应激过程中产生的活性氧在脊髓损伤的发病机制中起着重要作用。抗氧化剂莫诺苷是中药山茱萸的主要活性成分。近年来，有报道莫诺苷对氧化损伤介导的多器官损伤具有治疗作用，但莫诺苷对脊髓损伤的作用尚未见报道。因此，本研究的目的是评估莫诺苷对SCI的治疗效果，并通过SCI后立即直接灌胃给药来确定其潜在机制。我们的研究表明莫诺苷治疗可以改善SCI后大鼠的功能恢复。这种行为改善与SCI后神经元和少突胶质细胞的较高存活率相关，这增加了受损脊髓（SC）形成髓鞘和修复组织的能力，最终有助于改善神经功能结局。此外，我们的研究发现，氧自由基增加，抗氧化酶活性下降，在受伤的SC。有趣的是，莫诺苷治疗降低氧自由基水平，增加抗氧化酶活性。总之，我们的研究结果表明，莫诺苷可能是一种有效的治疗改善SCI后的结果，其抗氧化活性可能是莫诺苷发挥神经保护作用的机制之一。
Spinal cord injury (SCI) is a disabling condition that often leads to permanent neurological deficits without an effective treatment. Reactive oxygen species (ROS) produced during oxidative stress play a vital role in the pathogenesis following SCI. The antioxidant morroniside is the main active component of the Chinese medicine Cornus officinalis. In recent years, it has been reported that morroniside has therapeutic effects on damage to multiple organs mediated by oxidative damage, but the effect of morroniside on SCI has not been reported. The purpose of this study was therefore to assess the therapeutic effect of morroniside on SCI, and to identify its underlying mechanism by direct intragastric administration immediately after SCI. Our study showed that morroniside treatment improved the functional recovery of rats following SCI. This behavioral improvement was associated with the higher survival in neurons and oligodendrocytes following SCI, which increased the capacity of injured spinal cord (SC) to form myelin and repair tissue, eventually contributing to improved neurological outcome. Furthermore, our study found that oxygen free radicals increased and antioxidant enzyme activity decreased in the injured SC. Interestingly, morroniside treatment decreased oxygen free radical levels and increased antioxidant enzyme activities. Together, our results suggested that morroniside may be an effective treatment for improving outcomes following SCI, and that its antioxidant activity may be one of the mechanisms by which morroniside exerts neuroprotective effects on SCI.