A novel gene, msa1, inhibits sexual differentiation in Schizoscharomyces pombe
A novel gene, msa1, inhibits sexual differentiation in Schizoscharomyces pombe
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DOI：
10.1534/genetics.167.1.77
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发表时间：
2004-05-01
期刊：
影响因子：
3.3
通讯作者：
Kawamukai, M
中科院分区：
文献类型：
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作者：
Jeong, HT;Ozoe, F;Kawamukai, M
裂殖酵母粟酒裂殖酵母的性别分化是由营养饥饿或交配信息素的存在引发的。我们发现了一个新基因 msal，它编码一种 533 个氨基酸的假定 RNA 结合蛋白，可抑制性分化。 msa1 基因的破坏导致细胞产生过多孢子。氮饥饿数小时后，细胞内 msal RNA 和 Msal 蛋白水平下降。遗传分析表明msal的功能独立于cAMP通路和应激反应通路。在二倍体细胞中删除 rust 基因会抑制孢子形成，在单倍体细胞中会降低交配信息素诱导基因（如 mei2、mam2、ste11 和 rep1）的表达。同时删除 msal 会逆转两种表型。 msal 的过表达会降低激活的 Ras1(Val17) 诱导的 mam2 表达。仅删除 rad24 以及同时删除 msal 和 rad24 的细胞之间的表型超孢子形成相似，但同时删除 msal 和 msa2/nrd1 会进一步增加超孢子形成。因此，我们认为 Msal 的主要功能是通过控制 Ste11 调节基因的表达（可能通过信息素信号通路）来负向调节性分化。
Sexual differentiation in the fission yeast Schizosaccharomyces pombe is triggered by nutrient starvation or by the presence of mating pheromones. We identified a novel gene, msal, which encodes a 533-aa putative RNA-binding protein that inhibits sexual differentiation. Disruption of the msa1 gene caused cells to hypersporulate. Intracellular levels of msal RNA and Msal protein diminished after several hours of nitrogen starvation. Genetic analysis suggested that the function of msal is independent of the cAMP pathway and stress-responsive pathway. Deletion of the rust gene in diploid cells inhibited sporulation and in haploid cells decreased expression of mating-pheromone-induced genes such as mei2, mam2, ste11, and rep1; simultaneous deletion of msal reversed both phenotypes. Overexpression of msal decreased activated Ras1(Val17)-induced expression of mam2. Phenotypic hypersporulation was similar between cells with deletion of only rad24 and both msal and rad24, but simultaneous deletion of msal and msa2/nrd1 additively increased hypersporulation. Therefore, we suggest that the primary function of Msal is to negatively regulate sexual differentiation by controlling the expression of Ste11-regulated genes, possibly through the pheromone-signaling pathway.