A pair of partially overlapping Arabidopsis genes with antagonistic circadian expression.
A pair of partially overlapping Arabidopsis genes with antagonistic circadian expression.
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DOI：
10.1155/2012/349527
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Meyer P
中科院分区：
文献类型：
--
作者：
Kunova A;Zubko E;Meyer P
大量的植物基因与部分重叠的基因以反义方向进行比对。因此，这两种基因的转录将有利于双链RNA的形成，为RNAi机制提供底物，并且增强的反义转录将因此降低有义转录物水平。我们已经确定了一个类似于基于反义的基因调控模型的基因对，因为T-DNA插入到反义基因中会导致反义转录水平的降低和有义转录水平的增加。然而，当这两个基因作为转基因插入到不同的染色体位置时，也观察到相同的效果，而与正义和反义基因单独或联合表达无关。因此，我们的研究结果表明，正义/反义转录水平的拮抗性变化并不一定反映反义介导的调节。更有可能的是，这两个基因的部分重叠可能有利于对抗性表达模式的进化，从而防止RNAi效应。
A large number of plant genes are aligned with partially overlapping genes in antisense orientation. Transcription of both genes would therefore favour the formation of double-stranded RNA, providing a substrate for the RNAi machinery, and enhanced antisense transcription should therefore reduce sense transcript levels. We have identified a gene pair that resembles a model for antisense-based gene regulation as a T-DNA insertion into the antisense gene causes a reduction in antisense transcript levels and an increase in sense transcript levels. The same effect was, however, also observed when the two genes were inserted as transgenes into different chromosomal locations, independent of the sense and antisense gene being expressed individually or jointly. Our results therefore indicate that antagonistic changes in sense/antisense transcript levels do not necessarily reflect antisense-mediated regulation. More likely, the partial overlap of the two genes may have favoured the evolution of antagonistic expression patterns preventing RNAi effects.