Down-regulated expression of SATB2 is associated with metastasis and poor prognosis in colorectal cancer
Down-regulated expression of SATB2 is associated with metastasis and poor prognosis in colorectal cancer
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SATB2表达下调与结直肠癌转移和不良预后相关
DOI：
10.1002/path.2575
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发表时间：
2009-09-01
影响因子：
7.3
通讯作者：
Li, Jian-Ming
中科院分区：
文献类型：
--
作者：
Wang, Shuang;Zhou, Jun;Li, Jian-Ming
为了寻找结直肠癌转移的新生物标志物，我们建立了小鼠结直肠癌原位植入模型，并选择了体内M5，这是SW480结直肠癌细胞系的一个亚克隆，具有增强转移到肝脏的潜力。我们使用基因表达谱比较了M5和SW480细胞之间基因表达谱的差异。我们发现M5细胞中特殊的富含at的序列结合蛋白2 （SATB2）表达下调。146例结直肠肿瘤样本的免疫组化分析显示，SATB2低表达与结直肠癌预后不良、肿瘤侵袭、淋巴结转移、远处转移及Dukes分级密切相关。单因素和多因素生存分析进一步表明，SATB2表达是CRC的潜在有利预后因素。这些结果不仅表明SATB2是CRC的一个潜在的新预后因子，而且表明选择体内高转移性SW480克隆结合基因表达谱是识别CRC预后标志物的有力方法。版权所有(C) 2009大不列颠和爱尔兰病理学会。约翰·威利父子有限公司出版。
To identify novel biomarkers of metastasis of colorectal cancer (CRC), we developed an orthotopic implantation model of murine CRC and selected in vivo M5, a subclone of the SW480 CRC cell line with enhanced potential for metastasis to the liver. We compared the differences in the gene expression profiles between M5 and SW480 cells using gene expression profiling. We found that expression of special AT-rich sequence-binding protein 2 (SATB2) was down-regulated in M5 cells. Immunohistochemical analysis of 146 colorectal tumour samples showed that underexpression of SATB2 was strongly correlated with poor prognosis, tumour invasion, lymph node metastasis, distant metastasis, and Dukes' classification for CRC. Univariate and multivariate survival analyses further showed that SATB2 expression was a potential favourable prognostic factor for CRC. These results demonstrated not only that SATB2 is a potential novel prognostic factor for CRC, but also that selection of a highly metastatic clone of SW480 in vivo coupled with gene expression profiling is a powerful approach to identifying prognostic markers for CRC. Copyright (C) 2009 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.