Building an Antifouling Zwitterionic Coating on Urinary Catheters Using an Enzymatically Triggered Bottom-Up Approach
Building an Antifouling Zwitterionic Coating on Urinary Catheters Using an Enzymatically Triggered Bottom-Up Approach
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DOI：
10.1021/am501961b
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发表时间：
2014-07-23
影响因子：
9.5
通讯作者：
Tzanov, Tzanko
中科院分区：
文献类型：
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作者：
Diaz Blanco, Carlos;Ortner, Andreas;Tzanov, Tzanko
导尿管相关性尿路感染在住院期间很常见，因为留置装置上会形成细菌生物膜。在这项研究中，我们报告了一种基于生物技术的创新方法，用于将带有防污染两性部分的硅胶导管共价功能化，以防止生物被膜的形成。我们的方法结合了在漆酶引发的表面自由基聚合中生物催化偶联到磺基甜菜碱-丙烯酸残基的天然酚层的潜在生物活性。为了确保足够的涂层在尿液中的稳定性，硅胶导管是等离子激活的。与工业化学方法不同，含有甲基丙烯酸酯的两性离子单体在pH 5和50℃下聚合，使用漆酶在涂有酚类物质的导管表面单独产生的酚氧基作为引发剂。用X射线光电子能谱(XPS)、傅里叶变换红外光谱(FTIR)、原子力显微镜(AFM)和比色法对涂层导管进行了表征。接触角和蛋白质吸附测量，再加上静态和动态条件下的革兰氏阴性铜绿假单胞菌和革兰氏阳性金黄色葡萄球菌的体外测试，模拟导尿管面临的操作条件，显示与未经改良的导尿管相比，生物被膜的形成减少了约8096。两性离子涂层在7天内不影响人成纤维细胞(BJ-Sta)的活性，这与导尿管的使用寿命延长相对应。
Catheter associated urinary tract infections are common during hospitalization due to the formation of bacterial biofilms on the indwelling device. In this study, we report an innovative biotechnology-based approach for the covalent functionalization of silicone catheters with antifouling zwitterionic moieties to prevent biofilm formation. Our approach combines the potential bioactivity of a natural phenolics layer biocatalytically conjugated to sulfobetaine-acrylic residues in an enzymatically initiated surface radical polymerization with laccase. To ensure sufficient coating stability in urine, the silicone catheter is plasma-activated. In contrast to industrial chemical methods, the methacrylate-containing zwitterionic monomers are polymerized at pH 5 and 50 degrees C using as an initiator the phenoxy radicals solely generated by laccase on the phenolics-coated catheter surface. The coated catheters are characterized by X-ray photoelectron spectroscopy (XPS), Fourier transformed infrared (FTIR) analysis, atomic force microscopy (AFM), and colorimetrically. Contact angle and protein adsorption measurements, coupled with in vitro tests with the Gram-negative Pseudomonas aeruginosa and Gram-positive Staphylococcus aureus in static and dynamic conditions, mimicking the operational conditions to be faced by the catheters, demonstrate reduced biofilm formation by about 8096 when compared to that of unmodified urinary catheters. The zwitterionic coating did not affect the viability of the human fibroblasts (BJ-Sta) over seven days, corresponding to the extended useful life of urinary catheters.