Vertical sections of cultivated anchorage‐dependent cells for electron microscopy
Vertical sections of cultivated anchorage‐dependent cells for electron microscopy
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用于电子显微镜的培养贴壁依赖性细胞的垂直切片
DOI：
10.1111/j.1365-2818.1983.tb04178.x
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发表时间：
1983
影响因子：
2
通讯作者：
G. Vergani
中科院分区：
文献类型：
--
作者：
S. Pentz;S. Amthor;G. Vergani
本文描述了一种使用TCSC‐1测试室系统对垂直切片培养细胞进行电镜制备的方法。细胞在特殊的箔片上培养。它们可以固定并直接嵌入腔室中。树脂聚合后，箔可以很容易地取下，第二树脂层倒在嵌入的电池上。然后在倒置光学显微镜下标记出不同的细胞。树脂的双层结构为依赖于锚定的细胞的垂直切片提供了最佳条件。
A method is described for electron microscopic preparation of cultivated cells for vertical sections using the test chamber system TCSC‐1. The cells are cultivated on a special foil. They can be fixed and embedded directly in the chamber. After polymerization of the resin, the foil can be easily taken off and a second resin layer is poured upon the embedded cells. Then distinct cells can be marked under an inverted light microscope. The bilayer of the resin allows optimal conditions for vertical sections of anchorage‐dependent cells.