Muscle-specific expression of myogenin in zebrafish embryos is controlled by multiple regulatory elements in the promoter
Muscle-specific expression of myogenin in zebrafish embryos is controlled by multiple regulatory elements in the promoter
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DOI：
10.1016/s1096-4959(02)00194-x
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发表时间：
2003-01-01
影响因子：
2.2
通讯作者：
Anyangwe, V
中科院分区：
文献类型：
--
作者：
Du, SJ;Gao, J;Anyangwe, V
肌细胞生成素是基本的螺旋环抑制转录因子家族的成员，在成肌细胞的特化和分化中发挥关键作用。肌细胞生成素在斑马鱼胚胎体节和骨骼肌发育中特异表达。为了研究肌细胞生成素基因的表达调控，我们报道了斑马鱼肌细胞生成素基因的特征及其在斑马鱼胚胎中的启动子活性分析。我们的数据表明，0.8 kb的肌细胞生成素启动子足以指导正确的时间和空间肌肉特异性绿色荧光蛋白在斑马鱼胚胎中的表达。序列分析确定了两个推定的E盒位点的肌细胞生成素基因启动子。此外，在启动子中还发现了MEF2识别位点和MEF3结合位点。E盒、MEF2或MEF3结合位点的突变对肌细胞生成素启动子的肌肉特异性和活性几乎没有影响。然而，以各种组合突变这些位点，例如E盒和MEF2结合位点，或MEF2和MEF3位点显著降低了启动子的活性。此外，同时突变E盒、MEF 2和MEF 3位点几乎消除了启动子的活性。这些数据表明肌细胞生成素在斑马鱼胚胎中的肌肉特异性表达受启动子中的多个调控元件控制。此外，由于这些调控元件控制肌细胞生成素在小鼠和人类胚胎中的表达，这些数据表明，控制肌细胞生成素表达的调控机制可能在进化过程中是保守的。(C)2002年爱思唯尔科学公司All rights reserved.
Myogenin is a member of the basic Helix-Loop-Helix transcription factor family that play key roles in myoblast specification and differentiation. Myogenin is specifically expressed in developing somite and skeletal muscles in zebrafish embryos. To determine the regulation of myogenin expression, we reported here the characterization of zebrafish myogenin gene and analysis of its promoter activity in zebrafish embryos. Our data showed that a 0.8-kb myogenin promoter was sufficient to direct correct temporal and spatial muscle-specific green fluorescence protein expression in zebrafish embryos. Sequence analysis identified two putative E box sites in the myogenin gene promoter. In addition, a MEF2 recognition site and a MEF3 binding site were also found in the promoter. Mutation of the E boxes, MEF2 or MEF3 binding site individually had little effect on the muscle-specificity and activity of the myogenin promoter. However, mutating these sites in various combinations, e.g. E boxes and MEF2 binding site, or MEF2 and MEF3 sites significantly reduced the activity of the promoter. Moreover, mutating the E boxes, MEF2 and MEF3 sites together almost abolished the activity of the promoter. These data indicate that muscle-specific expression of myogenin in zebrafish embryos is controlled by multiple regulatory elements in the promoter. In addition, because these regulatory elements control myogenin expression in mouse and human embryos, these data suggest that the regulatory mechanism controlling myogenin expression might be conserved during evolution. (C) 2002 Elsevier Science Inc. All rights reserved.