Low diversity of T4-type bacteriophages in applied rice straw, plant residues and rice roots in Japanese rice soils: Estimation from major capsid gene (g23) composition
Low diversity of T4-type bacteriophages in applied rice straw, plant residues and rice roots in Japanese rice soils: Estimation from major capsid gene (g23) composition
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DOI：
10.1111/j.1747-0765.2010.00513.x
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发表时间：
2010-12-01
影响因子：
2
通讯作者：
Kimura, Makoto
中科院分区：
文献类型：
--
作者：
Fujihara, Shun;Murase, Jun;Kimura, Makoto
本研究通过分析水稻主衣壳基因（g23）的组成，阐明了施用稻草、土壤和水稻根中植物残体中T4型噬菌体群落的多样性。g23片段的变性梯度凝胶电泳（DGGE）带型简单，彼此相似，没有任何季节性变化。从施用稻草、植物残体和水稻根系中分别获得22个、20个和26个g23无性系。大多数g23克隆属于水稻组I，II，III，V和VIII的稻草样品，水稻组I，II和V的植物残留物样品，水稻组I，II，III，V，VI和VIII的水稻根样品，表明较低的多样性g23基因在这些网站比散装土壤。没有季节性变化的g23克隆的系统发育分布的记录，在这些网站的细菌群落的明确的季节性变化。
The present study elucidated the diversity of T4-type bacteriophage communities in applied rice straw and plant residues in soil and rice roots by analyzing the composition of the major capsid gene (g23). The denaturing gradient gel electrophoresis (DGGE) band patterns of g23 fragments were simple and similar to each other without any seasonal change. Twenty-two, twenty and twenty-six g23 clones were obtained from applied rice straw, plant residues and rice roots, respectively. Most of the g23 clones belonged to Paddy Groups I, II, III, V and VIII for rice straw samples, to Paddy Groups I, II and V for plant residue samples, and to Paddy Groups I, II, III, V, VI and VIII for rice root samples, indicating a lower diversity of g23 genes at these sites than in bulk soils. No seasonal variation in the phylogenetic distribution of g23 clones was recorded, in contrast to clear seasonal variations in the bacterial communities at these sites.