Biological Evaluation of the Pollution of the Tsurumi River with 7-Ethoxycoumarin O-Deethylase Activity Induced by River Sediment Extracts in HepG2 Cells
Biological Evaluation of the Pollution of the Tsurumi River with 7-Ethoxycoumarin O-Deethylase Activity Induced by River Sediment Extracts in HepG2 Cells
复制标题
河流沉积物提取物在 HepG2 细胞中诱导的 7-乙氧基香豆素 O-脱乙基酶活性对鹤见河污染的生物学评价
DOI：
10.1248/jhs.49.8
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发表时间：
2003
影响因子：
--
通讯作者：
Y. Inouye
中科院分区：
文献类型：
--
作者：
S. Nito;Yuichiro Kanno;A. Muto;A. Uesugi;T. Nakahama;Y. Inouye
利用河流沉积物提取物，利用芳香烃受体（AhR）诱导HepG2细胞7-乙氧基香豆素o -去乙基酶（ECOD）活性的方法，对流入东京湾的渡见河污染水平进行了研究。河流沉积物采样点分别为Namamugi、Kami-Sueyoshi、Tsunashima、新横滨A和B（分别位于原露天工业垃圾焚烧场的下游和上游）、Kozukue和Nakayama（图1）。所有样品的ECOD活性均受到不同程度的诱导。位于京新工业区中部的namamui被认为是污染最严重的，其次是新横滨A和海啸岛。这些样品的结果显示，在ECOD活性和3-甲基胆酸（3-MC）方面，尽管HepG2细胞中CYP1A1 mRNA的表达在较高浓度下与3-MC的情况大致保持不变，但在u型剂量-反应曲线中，ECOD活性和3-甲基胆酸（3-MC）的表达大致保持不变，这意味着转录后抑制。
The pollution level of the Tsurumi River flowing into Tokyo Bay was studied longitudinally using river sediment extracts in an assay system based on the arylhydrocarbon receptor (AhR)-dependent induction of 7-ethoxycoumarin O-deethylase (ECOD) activity in HepG2 cells. The sampling points of river sediment were as follows: Namamugi, Kami-Sueyoshi, Tsunashima, Shin-Yokohama A and B (downstream and upstream, respectively, from the former open-air industrial waste incineration site), Kozukue and Nakayama (Fig. 1). ECOD activity was induced to different extents in all samples tested. Namamugi, located in the middle of Keihin Industrial District, was considered to be the most polluted, followed by Shin-Yokohama A and Tsunashima. Results from these samples showed reverse U-shaped dose-response curves in terms of ECOD activity as well as 3-methylcholantrene (3-MC) although the expression of CYP1A1 mRNA in HepG2 cells remained roughly constant at higher concentrations as in the case of 3-MC, thus implying post-transcriptional suppression.