Nicotine and lipopolysaccharide stimulate the formation of osteoclast-like cells by increasing macrophage colony-stimulating factor and prostaglandin E2 production by osteoblasts
Nicotine and lipopolysaccharide stimulate the formation of osteoclast-like cells by increasing macrophage colony-stimulating factor and prostaglandin E2 production by osteoblasts
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DOI：
10.1016/j.lfs.2005.08.017
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发表时间：
2006-03-06
期刊：
影响因子：
6.1
通讯作者：
Maeno, M
中科院分区：
文献类型：
--
作者：
Tanaka, H;Tanabe, N;Maeno, M
一些研究表明牙周炎引起牙槽骨破坏的原因之一是牙菌斑中革兰氏阴性菌细胞壁中的脂多糖（LPS），吸烟可能是牙周炎发生和严重程度的一个重要危险因素。本研究旨在确定尼古丁和LPS对成骨细胞巨噬细胞集落刺激因子（M-CSF）、骨保护素（OPG）和前列腺素E-2 （PGE(2)）表达的影响，以及尼古丁和LPS对破骨细胞样细胞形成的间接影响。Saos-2细胞分别用10(-3)M尼古丁、1或10 μ g/ml LPS和10(-3)M尼古丁培养14天。采用实时荧光定量PCR和酶联免疫吸附法分别检测M-CSF和OPG基因和蛋白的表达。ELISA法检测PGE2的表达。利用抗酒石酸酸性磷酸酶（TRAP）染色的破骨细胞前体，用尼古丁和lps处理的Saos-2细胞和nf - κ B配体的可溶性受体激活剂（RANKL）在条件培养基中培养，估计破骨细胞样细胞的形成。与单独培养的细胞相比，尼古丁和LPS联合培养的细胞中M-CSF和PGE(2)的表达明显增加。在烟碱和LPS的培养初期，OPG的表达增加，但在培养后期，OPG的表达减少。在含有尼古丁和lps处理的Saos-2细胞（含可溶性RANKL）产生的M-CSF和PGE(2)的条件培养基中，与单独尼古丁处理的Saos-2细胞相比，破骨细胞前体的TRAP染色增加。这些结果表明，尼古丁和LPS通过增加M-CSF和PGE(2)的产生来刺激破骨细胞样细胞的形成，并且这种刺激比单独使用尼古丁治疗更大。(c) 2005爱思唯尔公司版权所有。
Several studies have indicated that one of the causes of alveolar bone destruction with periodontitis is lipopolysaccharide (LPS) from the cell wall of Gram-negative bacteria in plaque and that tobacco smoking may be an important risk factor for the development and severity of periodontitis. The present study was undertaken to determine the effect of nicotine and LPS on the expression of macrophage colony-stimulating factor (M-CSF), osteoprotegerin (OPG), and prostaglandin E-2 (PGE(2)) in osteoblasts, and the indirect effect of nicotine and LPS on the formation of osteoclast-like cells. Saos-2 cells were cultured with 10(-3) M nicotine, or 1 or 10 mu g/ml LPS and 10(-3) M nicotine, for up to 14 days. The gene and protein expression of M-CSF and OPG were determined using real-time PCR and ELISA, respectively. PGE2 expression was determined using ELISA. The formation of osteoclast-like cells was estimated using tartrate-resistant acid phosphatase (TRAP) staining of osteoclast precursors in culture with conditioned medium from nicotine and LPS-treated Saos-2 cells and the soluble receptor activator of NF-kappa B ligand (RANKL). M-CSF and PGE(2) expression increased markedly in cells cultured with nicotine and LPS compared with those cultured with nicotine alone. OPG expression increased in the initial stages of culture with nicotine and LPS but decreased in the later stages of culture. The conditioned medium containing M-CSF and PGE(2) produced by nicotine and LPS-treated Saos-2 cells with soluble RANKL increased the TRAP staining of osteoclast precursors compared with that produced by nicotine treatment alone. These results suggest that nicotine and LPS stimulate the formation of osteoclast-like cells via an increase in M-CSF and PGE(2) production and that the stimulation is greater than with nicotine treatment alone. (c) 2005 Elsevier Inc. All rights reserved.