Acetaminophen induces liver injury and depletes glutathione in mice brain: Prevention by Moringa oleifera extract
Acetaminophen induces liver injury and depletes glutathione in mice brain: Prevention by Moringa oleifera extract
复制标题
DOI：
10.1016/j.sajb.2019.08.037
复制
发表时间：
2020-03-01
影响因子：
3.1
通讯作者：
Nguelefack, T. B.
中科院分区：
文献类型：
--
作者：
Fotio, A. L.;Nguepi, M. S. D.;Nguelefack, T. B.
对乙酰氨基酚（APAP）过量是全球急性肝衰竭的主要原因之一。辣木作为食品添加剂和植物药具有抗氧化、镇痛、解热、抗炎、免疫调节和保肝作用。研究辣木水提物对醋氨酚所致小鼠急性肝损伤及脑组织谷胱甘肽耗竭的影响。辣木叶水提取物（100和200 mg/kg，p.o.），蒸馏水和抗坏血酸（50 mg/kg，p.o.）在对乙酰氨基酚（500 mg/kg，p.o.）之前1和12小时给予小鼠。APAP处理后6小时处死小鼠。评估天冬氨酸氨基转移酶（AST）、丙氨酸氨基转移酶（ALT）和碱性磷酸酶（ALP）血清活性;丙二醛（MDA）和亚硝酸盐肝脏水平、肿瘤坏死因子α（TNF-α或TNF）和白细胞介素-1 β（IL-1 β）血清水平。在脑和肝组织中评价还原型谷胱甘肽（GSH）。通过苏木精/伊红（H&E）染色进行肝切片的组织学分析。APAP给药诱导肝损伤，肝和脑GSH水平显著降低（P <0.01）。血清ALT、ALP、AST活性升高（P <0.05），MDA、亚硝酸盐含量升高（P <0.05），血清TNF、IL-1 β升高（P <0.05）。辣木提取物显著减轻肝损伤（P <0.05），抑制血清ALT、AST和ALP活性。TNF和IL-1 β血清水平、亚硝酸盐和MDA肝含量显著降低（P <0.05）。油茶提取物，与APAP处理的小鼠相比。M.油茶提取物对乙酰氨基酚引起的组织损伤（空泡化和炎症），M。油茶施用。本研究结果表明，M.油茶叶提取物强烈地保护小鼠免于对乙酰氨基酚肝毒性，表现出抗氧化和抗炎作用。本研究结果支持M.用于治疗肝脏疾病。(C)2019款萨博。Elsevier B. V.出版，保留所有权利。
Acetaminophen (APAP) overdose is among the leading causes of acute liver failure worldwide. Moringa oleifera is used as food additive and, as phytomedicine for its antioxidant, analgesic, anti-pyretic, anti-inflammatory, immuno-modulatory and hepatoprotective properties. The present study investigated the effect of Moringa oleifera aqueous extract on acetaminophen-induced acute liver injury and glutathione depletion in mice brain. Moringa oleifera leaf aqueous extract (100 and 200 mg/kg, p.o.), distilled water and ascorbic acid (50 mg/kg, p.o.) were administered to mice 1 and 12 h before acetaminophen (500 mg/kg, p.o.). Mice were sacrificed 6 h after APAP treatment. Aspartate aminotransferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (ALP) serum activities; malondialdehyde (MDA) and nitrite liver level, tumor necrosis factor alpha (TNF-alpha or TNF) and interleukin-1 beta (IL-1 beta) serum levels were assessed. Reduced glutathione (GSH) was evaluated in brain and liver tissues. Histological analyses of liver section were performed by hematoxylin/eosin (H&E) staining. APAP administration induced liver injury with significant (P < .01) depletion of liver and brain GSH level. ALT, ALP and AST serum activities (P < .05), MDA and nitrite liver content, TNF and IL-1 beta in the serum were increased (P < .05). Moringa oleifera extract significantly (P < .05) reduced liver injury, inhibiting ALT, AST and ALP serum activities. TNF and IL-1 beta serum levels, nitrite, and MDA liver content were significantly (P < .05) reduced by M. oleifera extract, compared to APAP-treated mice. GSH depletion in brain and liver was inhibited by M. oleifera extract. Histological injuries (vacuolization and inflammation) by acetaminophen were remarkably prevented by M. oleifera administration. Results of the present study revealed that M. oleifera leaf extract strongly protects mice against acetaminophen hepatotoxicity, exhibiting antioxidant and anti-inflammatory effects. The present results support ethnopharmacological uses of M. oleifera for the treatment of liver diseases. (C) 2019 SAAB. Published by Elsevier B.V. All rights reserved.