Protective effects of phillygenin against CCl4 induced hepatic injury in rat
Protective effects of phillygenin against CCl4 induced hepatic injury in rat
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DOI：
10.3969/j.issn.1001-1978.2015.03.026
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发表时间：
2015-03-01
影响因子：
--
通讯作者：
Yao Jing-chun
中科院分区：
文献类型：
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作者：
Feng Qin;Xia Wen-kai;Yao Jing-chun
目的研究根腐素对CCl4致大鼠急性肝损伤的保护作用。方法采用CCl4诱导急性肝损伤大鼠丙氨酸转氨酶模型。测定大鼠血清ALT、AST活性和总胆红素浓度。光镜下观察肝组织切片。同时检测肝脏SOD、GSH- px、GSH活性及MDA含量。结果在CCl4致大鼠急性肝损伤模型中，根瘤菌原(0.05、0.15、0.5 mg；kg(-1), sc)显著降低血清ALT、AST和TBIL水平。根瘤素能有效改善肝脏组织病变。此外，茶根茎原(0.05,0.15,0.5 mg。0.15和0.5 mg显著提高肝组织中SOD、GSH- px和GSH活性，降低MDA含量。kg（-1）的剂量降低了tnf - α的浓度。和引发。结论连翘原素对CCl4致大鼠急性肝损伤有保护作用。这可能与增加抗氧化能力，减少肝组织脂质过氧化和降低tnf - α和IL-8水平有关。
Aim To study the protective effects of phillygenin against acute liver injury induced by CCl4 in rats. Methods The were induced by CCl4, in alanine aminotransferase models of acute liver injury rats. The serum activities of (ALT), aspartate aminotransferase (AST) and concentration of total bilirubin (TBIL) were determined, respectively. Liver tissue sections were examined under a light microscope. SOD, GSH-Px and GSH activities and MDA content in liver were also detected. TNF-alpha and IL-8 concentrations were determined by ELISA Results In the model of acute liver injury induced by CCl4 in rats, phillygenin (0.05, 0.15, 0.5 mg . kg(-1), sc) markedly decreased the elevated serum levels of ALT, AST and TBIL. Phillygenin effectively improved the tissue lesion of liver. In addition phillygenin (0.05, 0.15, 0.5 mg . kg(-1), se) significantly increased SOD, GSH-Px and GSH activities, and decreased the content of MDA in liver tissue, 0.15 and 0.5 mg . kg(-1) of phillygenin decreased the concentrations of TNF-alpha. and IL-8. Conclusion Phillygenin has a protective effect on acute liver injury induced by CCl4 in rats. This might be associated with increasing antioxidative capacity, decreasing lipid peroxidation in liver tissue and reducing TNF-alpha and IL-8 levels.