Respiratory viruses augment the adhesion of bacterial pathogens to respiratory epithelium in a viral species- and cell type-dependent manner
Respiratory viruses augment the adhesion of bacterial pathogens to respiratory epithelium in a viral species- and cell type-dependent manner
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DOI：
10.1128/jvi.80.4.1629-1636.2006
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发表时间：
2006-02-01
影响因子：
5.4
通讯作者：
Adderson, EE
中科院分区：
文献类型：
--
作者：
Avadhanula, V;Rodriguez, CA;Adderson, EE
继发性细菌感染往往使呼吸道病毒感染复杂化，但病毒易患细菌疾病的机制尚不完全清楚。我们测定了呼吸道合胞病毒(RSV)、人类副流感病毒3(HPIV-3)和流感病毒感染对非分型流感嗜血杆菌和肺炎链球菌黏附呼吸道上皮细胞能力的影响，以及这些病毒如何改变这些细菌的已知受体的表达。所有病毒都增强了细菌对原代和永生化细胞系的粘附性。RSV和HPIV-3感染可增加原代支气管上皮细胞和A549细胞几种已知的致病菌受体的表达，但对原代小气道上皮细胞无影响。流感病毒感染不会改变受体的表达。副粘病毒通过上调真核细胞对这些病原体的受体来增加细菌对原代支气管上皮细胞和永生化细胞系的黏附，而这一机制在原代小气道上皮细胞和流感病毒感染中并不明显。呼吸道病毒促进细菌与呼吸道上皮细胞的黏附，这一过程可能会增加细菌的定植并导致疾病。这些研究突出了不同细胞类型对病毒感染的不同反应，在解释呼吸道细胞和病毒病原体之间相互作用的研究时，需要考虑这种差异。
Secondary bacterial infections often complicate respiratory viral infections, but the mechanisms whereby viruses predispose to bacterial disease are not completely understood. We determined the effects of infection with respiratory syncytial virus (RSV), human parainfluenza virus 3 (HPIV-3), and influenza virus on the abilities of nontypeable Haemophilus influenzae and Streptococcus pneumoniae to adhere to respiratory epithelial cells and how these viruses alter the expression of known receptors for these bacteria. All viruses enhanced bacterial adhesion to primary and immortalized cell lines. RSV and HPIV-3 infection increased the expression of several known receptors for pathogenic bacteria by primary bronchial epithelial cells and A549 cells but not by primary small airway epithelial cells. Influenza virus infection did not alter receptor expression. Paramyxoviruses augmented bacterial adherence to primary bronchial epithelial cells and immortalized cell lines by up-regulating eukaryotic cell receptors for these pathogens, whereas this mechanism was less significant in primary small airway epithelial cells and in influenza virus infections. Respiratory viruses promote bacterial adhesion to respiratory epithelial cells, a process that may increase bacterial colonization and contribute to disease. These studies highlight the distinct responses of different cell types to viral infection and the need to consider this variation when interpreting studies of the interactions between respiratory cells and viral pathogens.