Calcium release from ryanodine receptors in the nucleoplasmic reticulum
Calcium release from ryanodine receptors in the nucleoplasmic reticulum
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DOI：
10.1016/j.ceca.2005.09.010
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发表时间：
2006-01-01
期刊：
影响因子：
4
通讯作者：
Leite, MF
中科院分区：
文献类型：
--
作者：
Marius, P;Guerra, MT;Leite, MF
Ca 2+信号控制DNA合成和修复，基因转录以及细胞核内发生的其他细胞功能。核被膜可以储存Ca ~（2+）并通过三磷酸肌醇受体（InsP 3R）或兰尼碱受体（RyR）将其释放到核中。此外，许多细胞类型的细胞核内具有网状网络，并且该核浆网上的InsP 3 Rs允许局部亚核控制Ca 2+信号和Ca 2+依赖性核内事件。然而，目前还不清楚RyR是否同样表达在核质网上，并可以控制亚核Ca 2+信号。在这里，我们报告说，1型RyR的肌浆网的C2 C12细胞，骨骼肌衍生的细胞系的核内延伸表达。此外，在核质网区域中的笼状Ca 2+的双光子光释放诱发了细胞核内Ca 2+诱导的Ca 2+释放（CICR），这可以被RyR抑制剂丹曲林抑制。这些结果表明，核包膜的核内延伸具有功能性RyR，并提供了一种可能的机制，即表达RyR的细胞可以调节细胞核内离散区域的Ca 2+信号。(c)2005爱思唯尔有限公司保留所有权利。
Ca2+ signals control DNA synthesis and repair, gene transcription, and other cell functions that occur within the nucleus. The nuclear envelope can store Ca2+ and release it into the nucleus via either the inositol 1,4,5-trisphosphate receptor (InsP3R) or the ryanodine receptor (RyR). Furthermore, many cell types have a reticular network within their nuclei and InsP3Rs on this nucleoplasmic reticulum permit local subnuclear control of Ca2+ signals and Ca2+-dependent intranuclear events. However, it is unknown whether RyR similarly is expressed on the nucleoplasmic reticulum and can control subnuclear Ca2+ signals. Here we report that the type 1 RyR is expressed on intranuclear extensions of the sarcoplasmic reticulum of C2C12 cells, a skeletal muscle derived cell line. In addition, two-photon photorelease of caged Ca2+ in the region of the nucleoplasmic reticulum evoked Ca2+-induced Ca2+ release (CICR) within the nucleus, which could be suppressed by the RyR inhibitor dantrolene. These results show that intranuclear extensions of the nuclear envelope have functional RyR and provide a possible mechanism whereby cells expressing RyR can regulate Ca2+ signals in discrete regions within the nucleus. (c) 2005 Elsevier Ltd. All rights reserved.