Sustained increase in 1,2-diacylglycerol precedes DNA synthesis in epidermal-growth-factor-stimulated fibroblasts. Evidence for stimulated phosphatidylcholine hydrolysis.
Sustained increase in 1,2-diacylglycerol precedes DNA synthesis in epidermal-growth-factor-stimulated fibroblasts. Evidence for stimulated phosphatidylcholine hydrolysis.
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在表皮生长因子刺激的成纤维细胞中，1,2-二酰甘油的持续增加先于 DNA 合成。
DOI：
10.1042/bj2670501
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Raben,DM
中科院分区：
文献类型：
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作者：
Wright,TM;Shin,HS;Raben,DM
许多生长因子的一个共同特性是它们必须在DNA合成和细胞分裂发生之前存在几个小时。在此期间相关的细胞内信号定义不清。我们检查了表皮生长因子（EGF）刺激后IIC 9成纤维细胞中1，2-二酰基甘油的形成，发现这种脂质的质量在至少四个小时内保持升高。EGF刺激的1，2-二酰甘油产生和[3 H]胸苷掺入的浓度依赖性相似。磷脂代谢的研究强烈表明，磷脂酰胆碱是响应EGF产生的1，2-二酰甘油的来源。EGF没有刺激其他磷脂的水解，包括磷酸肌醇，也没有增加1，2-二酰基甘油的从头合成。这种模式的持续1，2-二酰基甘油形成磷脂酰胆碱可能是重要的促有丝分裂信号的EGF和潜在的其他生长因子。
A property common to many growth factors is that they must be present for several hours before the commitment to DNA synthesis and cell division occurs. The intracellular signals that are relevant during this period are poorly defined. We examined the formation of 1,2-diacylglycerol in IIC9 fibroblasts after stimulation with epidermal growth factor (EGF), and found that the mass of this lipid remained elevated for at least four hours. The concentration-dependence of EGF-stimulated 1,2-diacylglycerol production and [3H]thymidine incorporation were similar. Studies of phospholipid metabolism strongly suggested that phosphatidylcholine was the source of the 1,2-diacylglycerol generated in response to EGF. EGF did not stimulate the hydrolysis of other phospholipids, including the phosphoinositides, nor did it increase synthesis de novo of 1,2-diacylglycerol. This pattern of sustained 1,2-diacylglycerol formation from phosphatidylcholine may be important in the mitogenic signalling of EGF and potentially other growth factors.