EspP, a Type V-secreted serine protease of enterohaemorrhagic Escherichia coli O157:H7, influences intestinal colonization of calves and adherence to bovine primary intestinal epithelial cells
EspP, a Type V-secreted serine protease of enterohaemorrhagic Escherichia coli O157:H7, influences intestinal colonization of calves and adherence to bovine primary intestinal epithelial cells
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DOI：
10.1111/j.1574-6968.2007.00724.x
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发表时间：
2007-06-01
影响因子：
2.1
通讯作者：
Stevens, Mark P.
中科院分区：
文献类型：
--
作者：
Dziva, Francis;Mahajan, Arvind;Stevens, Mark P.
肠出血性大肠杆菌（EHEC）是一组在世界范围内具有重要意义的人畜共患病病原体。牛是一个关键的水库;然而，促进肠出血性大肠杆菌在牛肠道持续定植的分子机制尚不明确。大肠杆菌O 157：H7的大质粒编码几个推定的毒力因子。在这里，据报道，pO 157编码的V型分泌的丝氨酸蛋白酶EspP影响小牛的肠道定植。为了剖析减毒的基础，开发了牛原代直肠上皮细胞系。E. coli O 157：H7对该细胞的作用因espP突变而显著减弱，但在加入高度纯化的外源EspP后恢复。这项研究的数据增加了越来越多的证据表明，细胞毒素促进肠出血性大肠杆菌肠道定植。
Enterohaemorrhagic Escherichia coli (EHEC) comprise a group of zoonotic diarrhoeal pathogens of worldwide importance. Cattle are a key reservoir; however the molecular mechanisms that promote persistent colonization of the bovine intestines by EHEC are ill-defined. The large plasmid of EHEC O157:H7 encodes several putative virulence factors. Here, it is reported that the pO157-encoded Type V-secreted serine protease EspP influences the intestinal colonization of calves. To dissect the basis of attenuation, a bovine primary rectal epithelial cell line was developed. Adherence of E. coli O157:H7 to such cells was significantly impaired by espP mutation but restored upon addition of highly purified exogenous EspP. Data of this study add to the growing body of evidence that cytotoxins facilitate intestinal colonization by EHEC.