Developmental ability of embryos produced from oocytes with fragile oolemma by intracytoplasmic sperm injection
Developmental ability of embryos produced from oocytes with fragile oolemma by intracytoplasmic sperm injection
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DOI：
10.1007/s10815-016-0811-4
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发表时间：
2016-12-01
影响因子：
3.1
通讯作者：
Ezono, Yuji
中科院分区：
文献类型：
--
作者：
Mizobe, Yamato;Oya, Naoto;Ezono, Yuji
在对具有脆弱卵膜（脆弱卵母细胞）的卵母细胞进行胞浆内单精子注射 (ICSI) 时，注射时可能会发生破裂。在这项研究中，我们通过 ICSI 从具有脆弱且正常卵膜的卵母细胞（正常卵母细胞）中产生了胚胎，并比较了它们在体外受精和发育的能力。我们还研究了囊胚移植后脆性卵母细胞来源的胚胎能否着床，以确定是否应使用脆性卵母细胞进行辅助生殖技术治疗。将卵母细胞分为三组——不含脆性卵母细胞的周期中的正常卵母细胞（A组）、含有至少1个脆性卵母细胞的周期中的正常卵母细胞（B组）和脆性卵母细胞（C组），比较其ICSI后的受精能力和所得胚胎的发育能力。C组的受精率变性率（65.3 %）显着（P < 0.01）低于 A 组（84.6 %）和 B 组（86.9 %），C 组变性率（24.2 %）显着（P < 0.01）高于 A 组（0.71 %）和 B 组（0.28 %）。然而，不同组间胚胎的囊胚形成率（59.7-67.5%）没有显着差异。此外，C组囊胚移植后的妊娠率（50.0％）与A组（35.6％）和B组（45.8％）没有显着差异。脆弱卵母细胞ICSI后的受精能力低于正常卵母细胞，但所得胚胎具有与正常卵母细胞衍生胚胎相同的发育能力。
In intracytoplasmic sperm injection (ICSI) of oocytes with a fragile oolemma (fragile oocytes), breakage can occur at injection. In this study, we produced embryos from oocytes with a fragile and normal oolemma (normal oocytes) by ICSI and compared their ability to be fertilized and develop in vitro. We also investigated whether fragile oocyte-derived embryos could implant after blastocyst transfer to determine whether fragile oocytes should be used for assisted reproductive technology treatment.Oocytes were divided into three groups-normal oocytes from cycles containing no fragile oocytes (group A), normal oocytes from cycles containing at least one fragile oocyte (group B), and fragile oocytes (group C), and their fertilization abilities after ICSI and the developmental abilities of resultant embryos were compared.The fertilization rate in group C (65.3 %) was significantly (P < 0.01) lower than those in groups A (84.6 %) and B (86.9 %), and the degeneration rate in group C (24.2 %) was significantly (P < 0.01) higher than those in groups A (0.71 %) and B (0.28 %). However, there were no significant differences in the blastocyst formation rates (59.7-67.5 %) of embryos among the different groups. In addition, the pregnancy rate after transfer of blastocysts in group C (50.0 %) was not significantly different from those in groups A (35.6 %) and B (45.8 %).The fertilization ability after ICSI of fragile oocytes is lower than that of normal oocytes but the resultant embryos have the same developmental ability as those of normal oocyte-derived embryos.