An inexpensive, automation-friendly protocol for recovering high-quality DNA
An inexpensive, automation-friendly protocol for recovering high-quality DNA
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DOI：
10.1111/j.1471-8286.2006.01428.x
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发表时间：
2006-12-01
期刊：
影响因子：
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通讯作者：
Hebert, Paul D. N.
中科院分区：
文献类型：
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作者：
Ivanova, Natalia V.;Dewaard, Jeremy R.;Hebert, Paul D. N.
尽管商业试剂盒可用于自动提取DNA，但“手工”方案却不能。在这项研究中，我们提出了一种基于二氧化硅的方法，该方法灵敏、廉价且符合自动化要求。该协议的有效性现已在5000多个动物样本上进行了测试，结果高度阳性。
Although commercial kits are available for automated DNA extraction, 'artisanal' protocols are not. In this study, we present a silica-based method that is sensitive, inexpensive and compliant with automation. The effectiveness of this protocol has now been tested on more than 5000 animal specimens with highly positive results.