Simple molecular identification and free amino acid contents of Neoporphyra kitoi (Bangiales, Rhodophyta) with potential use as a new species for nori cultivation
Simple molecular identification and free amino acid contents of Neoporphyra kitoi (Bangiales, Rhodophyta) with potential use as a new species for nori cultivation
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DOI：
10.1007/s10811-022-02896-8
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发表时间：
2023-01
影响因子：
3.3
通讯作者：
K. Niwa;Hiroshi Iga;Yushi Shimada;H. Furuita;Takeshi Yamamoto
中科院分区：
文献类型：
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作者：
K. Niwa;Hiroshi Iga;Yushi Shimada;H. Furuita;Takeshi Yamamoto
我们用Neoporphyra kitoito的OHN-1菌株进行了初步的实验培养，证实了海苔养殖场配子体叶片的生长。成熟和未成熟的长条形叶片在紫菜养殖场生长，并被证实是N。kitoi基于叶绿体RUBISCO间隔区的核苷酸序列。此外，我们开发了PCR-RFLP分析，利用叶绿体和核DNA简单地区分N。kitoiandNeopyropia yezoensis，在日本栽培的物种，为确定许多样品之间的生长比较，在未来的实验栽培。此外，还比较了N. kitoiOHN-1和培养的Neopy。narawaensis）HG-4进行了比较。结果表明，OHN-1中四种主要氨基酸的含量显著高于HG-4，尤其是Ala。结果表明，OHN-1株为N. Kitoi具有作为高生长和更好风味的新栽培种的潜力。
We conducted preliminary experimental cultivation using the OHN-1 strain ofNeoporphyra kitoito confirm the growth of the gametophytic blades in the nori farm. The mature and immature long linear blades grew in the nori farm and were confirmed to beN. kitoibased on the nucleotide sequences of the chloroplast RUBISCO spacer region. Furthermore, we developed PCR–RFLP analyses using chloroplast and nuclear DNA to discriminate simply betweenN. kitoiandNeopyropia yezoensis, the species cultivated in Japan, for identifying many samples for growth comparisons between the two species during future experimental cultivation. In addition, four major FAA contents [alanine (Ala), aspartic acid (Asp), glutamic acid (Glu) and taurine (Tau)] were compared between the blades ofN. kitoiOHN-1 and cultivatedNeopy. yezoensis(Neopyropia yezoensisf.narawaensis) HG-4 under identical laboratory conditions. As a result, the contents of the four major FAAs, especially Ala, were significantly higher in OHN-1 than in HG-4. The results suggested that the OHN-1 strain ofN. kitoihas potential as a new cultivated species with high growth and a better flavor.