Application of desorption electrospray ionization mass spectrometry imaging in breast cancer margin analysis
Application of desorption electrospray ionization mass spectrometry imaging in breast cancer margin analysis
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DOI：
10.1073/pnas.1408129111
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发表时间：
2014-10-21
影响因子：
11.1
通讯作者：
Agar, Nathalie Y. R.
中科院分区：
文献类型：
--
作者：
Calligaris, David;Caragacianu, Diana;Agar, Nathalie Y. R.
在保乳手术中，区分肿瘤与正常乳腺组织是重要的一步。由于这种区别在手术环境中可能具有挑战性，当使用传统方法时，高达40%的患者需要额外的手术。在这里，我们提出了一个概念验证的研究，以确定使用解吸电喷雾电离质谱成像（MSI）的可行性，以确定和区分正常乳腺组织的肿瘤。我们发现，肿瘤边缘可以确定使用的空间分布和不同强度的不同脂质。几种脂肪酸，包括油酸，在癌组织中比在正常组织中更丰富。MSI的分子图像所描绘的癌边缘与组织学染色所获得的边缘一致。我们的研究结果证明了使用环境电离MSI对癌组织和正常乳腺组织进行分类的可行性。结果表明，基于MS的方法可以开发用于在保乳手术中快速术中检测残留癌组织。
Distinguishing tumor from normal glandular breast tissue is an important step in breast-conserving surgery. Because this distinction can be challenging in the operative setting, up to 40% of patients require an additional operation when traditional approaches are used. Here, we present a proof-of-concept study to determine the feasibility of using desorption electrospray ionization mass spectrometry imaging (DESI-MSI) for identifying and differentiating tumor from normal breast tissue. We show that tumor margins can be identified using the spatial distributions and varying intensities of different lipids. Several fatty acids, including oleic acid, were more abundant in the cancerous tissue than in normal tissues. The cancer margins delineated by the molecular images from DESI-MSI were consistent with thosemargins obtained fromhistological staining. Our findings prove the feasibility of classifying cancerous and normal breast tissues using ambient ionization MSI. The results suggest that an MS-based method could be developed for the rapid intraoperative detection of residual cancer tissue during breast-conserving surgery.