Effects of phenanthraquinone on allergic airway inflammation in mice
Effects of phenanthraquinone on allergic airway inflammation in mice
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DOI：
10.1111/j.1365-2222.2005.02297.x
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发表时间：
2005-09-01
影响因子：
6.1
通讯作者：
Kumagai, Y
中科院分区：
文献类型：
--
作者：
Hiyoshi, K;Takano, H;Kumagai, Y
背景技术柴油机排气颗粒（DEP）增强小鼠的过敏性气道炎症（Takano等人，Am J Respir Crit Care Med 1997; 156：36-42）。DEP由碳质核和大量有机化合物组成。然而，它仍然有待确定哪些组件（S）从DEP负责的增强效果。9，10-菲醌（PQ）是一种参与DEP的醌类化合物。目的观察PQ对卵清蛋白（OVA）致敏小鼠气道炎症的影响。材料与方法观察PQ对OVA致敏和不致敏小鼠气道炎症、局部细胞因子蛋白表达和变应原特异性免疫球蛋白产生的影响。结果在OVA致敏的情况下，PQ（2.1 ng/动物）显着增加支气管肺泡灌洗液中的嗜酸性粒细胞和单核细胞的数量相比，单独的卵清蛋白。相反，在肺组织学中，在OVA激发和OVA + PQ激发之间，气道周围的这些细胞的数量没有显著差异。PQ表现出佐剂活性的过敏原特异性生产的IgG 1和IgE。与溶媒激发相比，OVA激发诱导肺中IL-4、IL-5、嗜酸性粒细胞趋化因子、巨噬细胞趋化蛋白-1和角质形成细胞趋化因子表达显著增加。结论PQ可促进免疫球蛋白的产生和炎症细胞向肺泡腔的浸润，这与OVA有关，而PQ可能是DEP致过敏性气道炎症毒性的部分原因。
Backgroundd Diesel exhaust particles (DEP) enhance allergic airway inflammation in mice (Takano et al., Am J Respir Crit Care Med 1997; 156: 36-42). DEP consist of carbonaceous nuclei and a vast number of organic chemical compounds. However, it remains to be identified which component(s) from DEP are responsible for the enhancing effects. 9,10-Phenanthraquinone (PQ) is a quinone compound involved in DEP.Objective To investigate the effects of PQ inoculated intratracheally on allergic airway inflammation related to ovalbumin (OVA) challenge.Materials and Methods We evaluated effects of PQ on airway inflammation, local expression of cytokine proteins, and allergen-specific immunoglobulin production in mice in the presence or absence of OVA.Results In the presence of OVA, PQ (2.1 ng/animal) significantly increased the numbers of eosinophils and mononuclear cells in bronchoalveolar lavage fluid as compared with OVA alone. In contrast, the numbers of these cells around the airways were not significantly different between OVA challenge and OVA plus PQ challenge in lung histology. PQ exhibited adjuvant activity for the allergen-specific production of IgG1 and IgE. OVA challenge induced significant increases in the lung expression of IL-4, IL-5, eotaxin, macrophage chemoattractant protein-1, and keratinocyte chemoattractant as compared with vehicle challenge. However, the combination of PQ with OVA did not alter the expression levels of these proteins as compared with OVA alone.Conclusion These results indicate that PQ can enhance the immunoglobulin production and the infiltration of inflammatory cells into alveolar spaces that are related to OVA, whereas PQ seems to be partially responsible for the DEP toxicity on the allergic airway inflammation.