Hepatitis C Virus Subtypes Circulating Among Intravenous Drug Users in Lisbon, Portugal
Hepatitis C Virus Subtypes Circulating Among Intravenous Drug Users in Lisbon, Portugal
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DOI：
10.1002/jmv.21955
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发表时间：
2011-04-01
影响因子：
12.7
通讯作者：
Esteves, Aida
中科院分区：
文献类型：
--
作者：
Calado, Rita Almeida;Rocha, Maria Raquel;Esteves, Aida
丙型肝炎病毒（HCV）感染了世界人口的2-3%，静脉注射吸毒是工业化国家传播的主要原因。由于葡萄牙HCV感染分子流行病学数据的缺乏，促使人们对居住在里斯本市区静脉吸毒者中传播的HCV亚型进行研究，采样间隔约10年（1998-2000年和2008-2009年）。从124例单独感染和合并感染的HCV病毒血症患者中获得了E1和/或NS5B的部分编码序列。系统发育分析表明，在第一次和第二次采样期间，la和3a亚型分别占64.9%和71.6%，在两个时间段内最流行。然而，通过比较亚型内遗传距离推断，较晚引入的基因型4病毒（亚型4a和4d）即使在十年前也相对频繁（24.6%）。葡萄牙静脉注射吸毒者的HCV亚型特征与其他南欧国家在与药物消费相关时的描述一致。除1b亚型外，系统发育树没有显示葡萄牙序列的聚类，而是显示来自不同地理起源的HCV序列的系统发育混合，正如之前在其他西方国家所描述的那样，这表明存在一个庞大的国际传播网络。与报道的HCV低重组率一致，只有一个样本显示两个分析区域的亚型不一致（El为4d， NS5B为4a），这表明可能存在新的重组，值得进一步分析。中华医学杂志，2011,31(3):444 - 444。(C) 2011 Wiley-Liss, Inc。
Hepatitis C virus (HCV) infects 2-3% of the world population and intravenous drug consumption is the leading cause of transmission in industrialized countries. The unavailability of data on the molecular epidemiology of HCV infection in Portugal prompted the study of HCV subtypes circulating among intravenous drug users residing in the Lisbon metropolitan area and sampled about 10 years apart (1998-2000 and 2008-2009). Partial coding sequences for E1 and/or NS5B were obtained from 124 individuals with HCV viremia, both mono-infected and co-infected with HIV. Phylogenetic analysis showed that, for both time periods, the most prevalent subtypes were la and 3a, found, altogether, in 64.9% and 71.6% of the individuals, respectively for the first and the second sampling periods. However, genotype 4 viruses (subtypes 4a and 4d), introduced later, as inferred by comparison of intra-subtype genetic distances, were also relatively frequent even one decade ago (24.6%). This HCV subtype profile for Portuguese intravenous drug users is in agreement with those described for other southern European countries when in association with drug consumption. With the exception of subtype 1b, phylogenetic trees did not show clustering of the Portuguese sequences, but rather phylogenetic mixing of HCV sequences from different geographic origins, as described previously in other Western countries and suggestive of a large international transmission network. Consistent with the low recombination rates reported for HCV, only one sample revealed discordant subtypes for the two regions analyzed (4d in El and 4a in NS5B), representing a potential new recombinant that deserves further analysis. J. Med. Virol. 83:608-615, 2011. (C) 2011 Wiley-Liss, Inc.