The effect of low-level laser irradiation on hyperglycemia-induced inflammation in human gingival fibroblasts
The effect of low-level laser irradiation on hyperglycemia-induced inflammation in human gingival fibroblasts
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DOI：
10.1007/s10103-018-2675-6
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发表时间：
2019-07-01
影响因子：
2.1
通讯作者：
Wang, Yan-Hsiung
中科院分区：
文献类型：
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作者：
Lee, Kun-Tsung Denzel;Chiang, Min-Hsuan;Wang, Yan-Hsiung
高血糖引起的炎症会大大增加糖尿病患者患牙周病的风险。低水平激光照射（LLLI）已被用于伤口愈合和抗炎在许多情况下，LLLI是已知的抑制脂多糖（LPS）刺激的炎症反应。然而，LLLI在糖尿病牙周炎患者中的治疗效果尚不清楚。在这项研究中，我们培养人牙龈成纤维细胞（HGF）在高糖培养基（35 mM）模拟高血糖的环境，然后测量LLLI的抗炎作用，通过评估促炎基因的表达，包括肿瘤坏死因子-α（TNF-α），白细胞介素（IL）-1 β，IL-6，IL-8的定量实时聚合酶链反应。结果表明，在甘露醇培养基中培养的HGF和仅用LLLI处理的HGF中没有显著的炎症反应。然而，仅在高葡萄糖培养基中培养的HGF显示出比在与LLLI一起处理的HGF中显着更高的促炎细胞因子表达。然后，我们观察到LLLI通过调节cAMP信号传导减少在高糖培养基中培养的HGF中促炎细胞因子的表达。我们还研究了抗氧化剂（维生素C）治疗是否降低了在高糖培养基中培养的HGF中氧化应激的炎症效应，但发现与LLLI共同治疗后没有累加效应，这表明LLLI可能激活cAMP信号传导，但不激活活性氧（ROS）信号传导，以减少高糖诱导的炎症。总之，LLLI可能对高糖环境中的HGF具有抗炎作用，并可能有益于糖尿病患者牙周病的治疗。
Hyperglycemia-induced inflammation can greatly increase the risk of periodontal disease in people with diabetes. Low-level laser irradiation (LLLI) has been used for wound healing and anti-inflammation in many cases, and LLLI is known to inhibit the lipopolysaccharide (LPS)-stimulated inflammatory response. However, the therapeutic effect of LLLI in diabetes patients with periodontitis remains unknown. In this study, we cultured human gingival fibroblasts (HGFs) in high-glucose medium (35 mM) to mimic a hyperglycemic environment, and then measured the anti-inflammatory effect of LLLI by assessing the expression of pro-inflammatory genes including tumor necrosis factor-alpha (TNF-alpha), interleukin (IL)-1 beta, IL-6, and IL-8 by quantitative real-time polymerase chain reaction. The results demonstrated no significant inflammatory response in HGFs cultured in mannitol medium and in those treated only with LLLI. However, HGFs cultured only in high-glucose medium showed significantly higher expression of pro-inflammatory cytokine than in those treated together with LLLI. We then observed that LLLI reduced the expression of pro-inflammatory cytokines in HGFs cultured in high-glucose medium by modulating cAMP signaling. We also investigated whether antioxidant (vitamin C) treatment reduced the inflammatory effect of oxidative stress in HGFs cultured in high-glucose medium but found no additive effect upon co-treatment with LLLI, suggesting that LLLI may activate cAMP signaling, but not reactive oxygen species (ROS) signaling, to reduce the high glucose-induced inflammation. In conclusion, LLLI may have an anti-inflammatory effect on HGFs in a high glucose environment and may benefit the treatment of periodontal disease in diabetes patients.