Effect of increasing sea water temperature on the growth and toxin production of the benthic dinoflagellate Prorocentrum lima
Effect of increasing sea water temperature on the growth and toxin production of the benthic dinoflagellate Prorocentrum lima
复制标题
DOI：
10.1007/s10750-018-3512-4
复制
发表时间：
2018-05-01
期刊：
影响因子：
2.6
通讯作者：
Morris, Steve
中科院分区：
文献类型：
--
作者：
Aquino-Cruz, Aldo;Purdie, Duncan A.;Morris, Steve
在未来几十年，由于全球气温升高（变暖），水生环境中有害藻类大量繁殖的频率可能会增加。本研究测试的假设，即细胞生长速率和盾叶甲贝类中毒（DSP）毒素（冈田酸，OA;甲藻毒素1，DTX 1）每细胞的底栖甲藻利马原甲藻增加与温度。利马青霉细胞在f/2培养基中生长，辐照度为50 +/- 15 A μ mol m（-2）s（-1），光周期为12 h L：12 h D。测定了细胞丰度、光合效率（Fv/Fm）和营养消耗（NO3（-）+ NO2（-）和PO 4（3-））。利马原螯虾的最适生长温度为15和25 ℃，但最高Fv/Fm值与最大生长速率无关。在5和30 A ℃下，利马青霉显示出较低的细胞生长速率和Fv/Fm值。只有每个细胞的游离OA浓度显示出随温度升高至15 A ℃而增加。在低温（10- 15 A ℃）和高温（30 A ℃）下的稳定生长期，发现利马青霉的亲脂性毒性最高。这项研究的结果表明，未来沿海沃茨气候条件的变化可能会导致世界范围内利马原螯虾种群的生长速度和细胞毒素水平升高。
In future decades, harmful algae blooms may increase in frequency in aquatic environments as a result of higher global temperatures (warming). This study tested the hypothesis that cell growth rate and Diarrethic Shellfish Poisoning (DSP) toxin (okadaic acid, OA; dinophysistoxin 1, DTX1) per cell of the benthic dinoflagellate Prorocentrum lima increases with temperature. P. lima cells were grown in f/2 medium at irradiances of 50 +/- 15 A mu mol m(-2) s(-1) and photoperiod of 12 h L:12 h D. Cell abundance, photosynthetic efficiency (Fv/Fm), and nutrient consumption (NO3 (-) + NO2 (-) and PO4 (3-)) were also determined. P. lima optimum growth temperature was at 15 and 25A degrees C but the highest Fv/Fm values showed no association to the maximum growth rate. P. lima showed lower cell growth rates and Fv/Fm values at both 5 and 30A degrees C. Only free OA concentration per cell showed an increase with temperature up to 15A degrees C. Highest lipophilic toxicity in P. lima was found during the stationary growth phase at low (10-15A degrees C) and elevated (30A degrees C) temperatures. Results from this study suggest that future changes to climatic conditions in coastal waters may lead to higher growth rates and cellular toxin levels in P. lima populations worldwide.