Ribosomal proteins at the stalk region modulate functional rRNA structures in the GTPase center
Ribosomal proteins at the stalk region modulate functional rRNA structures in the GTPase center
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DOI：
10.1074/jbc.m207424200
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发表时间：
2002-11-01
影响因子：
4.8
通讯作者：
Hachimori, A
中科院分区：
文献类型：
--
作者：
Uchiumi, T;Honma, S;Hachimori, A
用相应的大鼠对应物P0-P1/P2和真核L12替换大肠杆菌核糖体中的L10.L7/L12蛋白复合物和L11，导致核糖体对延伸因子的特异性从原核延伸因子（EF）-Tu/EF-G转化为真核EF（eEF）-1 α/eEF-2。在这里，我们研究了蛋白质替代对23 S rRNA的1070和2660位（八叠球菌素/蓖麻毒素环）附近的两个rRNA结构域的结构和功能的影响。在E. coli 50 S亚基的化学探针分析结果表明，大鼠蛋白质与1070区域的结合导致2660和1070区域对真核核糖体配体的可接近性增加：2660区域的核糖毒素pepocin（E. coli编号），抗28 S自身抗体用于1070区，eEF-2用于两个区。此外，E. coliL 10-L7/L12复合物和L11对1070区的作用。coli核糖体对另一种核糖毒素gypsophilin的可接近性。在1070区域的核糖体蛋白似乎以略微不同的模式在原核生物和真核生物中调节2660和1070 RNA区域的结构和功能。
Replacement of the L10.L7/L12 protein complex and L11 in Escherichia coli ribosomes with the respective rat counterparts P0-P1/P2 and eukaryotic L12 causes conversion of ribosomal specificity for elongation factors from prokaryotic elongation factor (EF)-Tu/EF-G to eukaryotic EF (eEF)-1alpha/eEF-2. Here we have investigated the effects of protein replacement on the structure and function of two rRNA domains around positions 1070 and 2660 (sarcin/ricin loop) of 23 S rRNA. Protein replacement at the 1070 region in E. coli 50 S subunits was demonstrated by chemical probing analysis. Binding of rat proteins to the 1070 region caused increased accessibility of the 2660 and 1070 regions to ligands for eukaryotic ribosomes: the ribotoxin pepocin for the 2660 region (E. coli numbering), anti-28 S autoantibody for the 1070 region, and eEF-2 for both regions. Moreover, binding of the E. coli L10-L7/L12 complex and L11 to the 1070 region was shown to be responsible for E. coli ribosomal accessibility to another ribotoxin, gypsophilin. Ribosomal proteins at the 1070 region appear to modulate the structures and functions of the 2660 and 1070 RNA regions in slightly different modes in prokaryotes and eukaryotes.