Spontaneously formed tumorigenic hybrids of Meth A sarcoma cells and macrophages in vivo
Spontaneously formed tumorigenic hybrids of Meth A sarcoma cells and macrophages in vivo
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DOI：
10.1002/ijc.11210
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发表时间：
2003-08-20
影响因子：
6.4
通讯作者：
Seljelid, R
中科院分区：
文献类型：
--
作者：
Busund, LTR;Killie, MK;Seljelid, R
我们最近证明了恶性细胞可以在体外与组织巨噬细胞杂交，产生致瘤性杂交。我们现在证明，这种情况可以在体内自发发生，因为接种的甲状肉瘤细胞与宿主细胞（可能是巨噬细胞）融合。因此，从胶原酶灌注和密度离心制备的肿瘤细胞悬液中，可以分离出具有肿瘤性但与甲基A表达的巨噬细胞标志物相反且具有吞噬能力的杂交细胞。其形态特征介于甲氧甲胺素A和巨噬细胞之间。利用半同种异体实验系统，将甲基a细胞从BALB/c （H-2 K-d）接种到（BALB）。K x BALB/c) F-1 (H-2(K /d))，来自这些肿瘤的杂交细胞可以表达甲基A和宿主单倍型的MHC抗原。杂交细胞在体内的生长速度比甲基A细胞快，表明通过异型细胞融合获得了促进生长的特性。(C) 2003 Wiley-Liss。公司。
We have recently demonstrated that malignant cells can hybridize with tissue macrophages in vitro, giving rise to tumorigenic hybrids. We now demonstrate that this can occur spontaneously in vivo as a result of fusion between inoculated Meth A sarcoma cells and host cells, presumably macrophages. Thus, from tumor cell suspensions prepared by collagenase perfusion and density centrifugation, hybrid cells could be isolated that were neoplastic but in contrast to Meth A expressed macrophage markers and had phagocytic capacity. Their morphologic features were intermediate between Meth A and macrophages. By taking advantage of a semiallogeneic experimental system by inoculation of Meth A cells from BALB/c (H-2 K-d) into (BALB.K x BALB/c) F-1 (H-2(k/d)), hybrid cells from these tumors could be shown to express MHC antigens of both the Meth A and the host haplotypes. Hybrid cells grew faster than Meth A cells in vivo, indicating acquisition of growth-promoting properties through heterotypic cell fusion. (C) 2003 Wiley-Liss. Inc.