THE SINGLE PSEUDOURIDINE RESIDUE IN ESCHERICHIA-COLI 16S RNA IS LOCATED AT POSITION-516
THE SINGLE PSEUDOURIDINE RESIDUE IN ESCHERICHIA-COLI 16S RNA IS LOCATED AT POSITION-516
复制标题
DOI：
10.1093/nar/22.18.3681
复制
发表时间：
1994-09-11
影响因子：
14.9
通讯作者：
OFENGAND, J
中科院分区：
文献类型：
--
作者：
BAKIN, A;KOWALAK, JA;OFENGAND, J
采用直接和间接相结合的方法测定了大肠杆菌16S核糖体RNA中假尿嘧啶残基的数量和位置。只在516位发现了一个残留物。这个位点位于RNA分子三个高度保守的长序列之一的5'端。许多实验结果强烈地暗示了这个环在A位点结合的tRNA识别密码子的保真度。由于它的位置，我们认为psi516也可能在维持蛋白质合成的保真度方面发挥作用。
The number and location of pseudouridine residues in Escherichia coli 16S ribosomal RNA has been determined by a combination of direct and indirect methods. Only one residue was found, at position 516. This site is at the 5'-end of one of the three most highly conserved long sequences of this RNA molecule. A number of experimental findings have strongly implicated this loop in the fidelity of codon recognition by A-site bound tRNA. By virtue of its location, we suggest that Psi 516 may also play a role in maintaining the fidelity of protein synthesis.