Telomerase activity in hematopoietic cells is associated with self-renewal potential
Telomerase activity in hematopoietic cells is associated with self-renewal potential
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DOI：
10.1016/s1074-7613(00)80316-7
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发表时间：
1996-09-01
期刊：
影响因子：
32.4
通讯作者：
Weissman, IL
中科院分区：
文献类型：
--
作者：
Morrison, SJ;Prowse, KR;Weissman, IL
有人提出，真核细胞分裂潜力有限的生物钟是端粒长度。我们检测了造血和免疫系统中单个细胞的端粒酶活性。我们检测了处于四个分化阶段的造血干细胞、定向祖细胞、成熟的髓系细胞和淋巴系细胞。每个群体中表达端粒酶的细胞的频率与被认为具有自我更新潜力的细胞的频率成正比。在骨髓造血干细胞中，70%显示出可检测到的端粒酶活性。本研究中观察到的表达端粒酶的体细胞并不被认为是永生的，并且表达与细胞周期分布或分化状态无关。这项研究表明，与端粒酶表达最一致的发育特征是自我更新潜力。
It has been proposed that the biological clock underlying the limited division potential of eukaryotic cells is telomere length. We assayed telomerase activity in single cells of the hematopoietic and immune systems. We examined hematopoietic stem cells at four stages of differentiation, lineage-committed progenitors, and mature myeloid and lymphoid cells. The frequency of telomerase-expressing cells within each population was proportional to the frequency of cells thought to have self-renewal potential. Among bone marrow hematopoietic stem cells, 70% exhibited detectable telomerase activity. The telomerase-expressing somatic cells observed in this study are not thought to be immortal, and expression was not correlated with cell cycle distribution or differentiation state. This study demonstrates that the developmental characteristic most consistently associated with telomerase expression is self-renewal potential.