CD8+ T-cell priming during a central nervous system infection with mouse hepatitis virus.
CD8+ T-cell priming during a central nervous system infection with mouse hepatitis virus.
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DOI：
10.1007/978-0-387-33012-9_68
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发表时间：
2006
影响因子：
--
通讯作者：
Weiss SR
中科院分区：
文献类型：
--
作者：
MacNamara KC;Weiss SR
1. INTRODUCTION study central nervous system (CNS) diseases including both encephalitis and demyelination. Different strains of MHV induce disease with varying degrees of severity. The A59 strain induces acute encephalitis during the first week of infection and a strong CD8+ T-cell response is observed in the brain coinciding with virus clearance. Despite efficient clearance of infectious virus, demyelination is evident four weeks postinfection (pi). The JHM strain (also referred to as MHV-4 or JHM. SD in the literature) 1 induces lethal encephalomyelitis within the first week of infection and virus is typically not cleared. In this study, we investigate the CD8+ T-cell responses induced during infections with A59 and JHM.Virus specific CD8+ T cells play a protective role against MHV strain A59 and are essential for clearance of infectious virus from the central nervous system (CNS). We have previously found that only early transfer, prior to 3 days postinfection (pi) with RA59-gfp/gp33, of gp33-specific CD8+ T cells (obtained from P14 transgenic mice) resulted in accumulation of activated epitope-specific CD8+ T cells within the brain. 2 We observed that P14 splenocytes did not accumulate in the brains of RA59-gfp/gp33 infected mice when the transfers were performed on day 3 or 5 pi In order to determine if this was due to a defect in trafficking or priming during the infection, we examined the expansion of transferred CFSE-labeled gp33-specific CD8+ T cells in the draining cervical lymph nodes following infection with RA59-gfp/gp33. In addition, we sought to determine why activated, virus-specific CD8+ T cells are detected at very low levels in the spleen and brain after infection with RJHM.
