The Long Noncoding RNA Landscape of the Ischemic Human Left Ventricle.
The Long Noncoding RNA Landscape of the Ischemic Human Left Ventricle.
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DOI：
10.1161/circgenetics.116.001534
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发表时间：
2017-01
期刊：
影响因子：
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通讯作者：
Muehlschlegel JD
中科院分区：
文献类型：
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作者：
Saddic LA;Sigurdsson MI;Chang TW;Mazaika E;Heydarpour M;Shernan SK;Seidman CE;Seidman JG;Aranki SF;Body SC;Muehlschlegel JD
长链非编码RNA（lncRNA）功能类别的发现扩大了我们对细胞中存在的各种RNA种类的理解。在心脏中，lncRNA涉及发育、缺血性和扩张性心肌病以及心肌梗死的调节。然而，有一个有限的描述，这些转录本在人类受试者的表达谱。我们从接受心脏手术的人类患者中获得左心室组织，并使用RNA测序来描述lncRNA谱。然后，我们确定了一系列lncRNA，这些lncRNA在心肺转流的缺血性损伤之前和之后的成对样品之间差异表达。这些lncRNA中的一些的表达与缺血时间相关。与差异表达的lncRNA非常接近的编码基因以及与这些lncRNA协调表达的编码基因在与心肌梗死相关的功能类别中富集，包括：心脏功能、代谢、应激反应和免疫系统。我们描述了一个清单的lncRNA，是差异表达后，在人类心脏缺血。预计这些基因在与心肌损伤一致的途径中发挥作用。因此，lncRNA可能成为缺血性心脏病诊断和治疗的新靶点。https：//clinicaltrials.gov/;唯一标识符：NCT 00985049。
The discovery of functional classes of long noncoding RNAs (lncRNAs) has expanded our understanding of the variety of RNA species that exist in cells. In the heart, lncRNAs have been implicated in the regulation of development, ischemic and dilated cardiomyopathy, and myocardial infarction. Nevertheless, there is a limited description of expression profiles for these transcripts in human subjects. We obtained left ventricular tissue from human patients undergoing cardiac surgery and used RNA sequencing to describe a lncRNA profile. We then identified a list of lncRNAs that were differentially expressed between pairs of samples before and after the ischemic insult of cardiopulmonary bypass. The expression of some of these lncRNAs correlates with ischemic time. Coding genes in close proximity to differentially expressed lncRNAs as well as coding genes that have coordinated expression with these lncRNAs are enriched in functional categories related to myocardial infarction including: heart function, metabolism, the stress response, and the immune system. We describe a list of lncRNAs that are differentially expressed after ischemia in the human heart. These genes are predicted to function in pathways consistent with myocardial injury. As a result, lncRNAs may serve as novel diagnostic and therapeutic targets for ischemic heart disease. https://clinicaltrials.gov/; Unique Identifier: NCT00985049.