Physical forces during collective cell migration
Physical forces during collective cell migration
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DOI：
10.1038/nphys1269
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发表时间：
2009-06-01
期刊：
影响因子：
19.6
通讯作者：
Fredberg, Jeffrey J.
中科院分区：
文献类型：
--
作者：
Trepat, Xavier;Wasserman, Michael R.;Fredberg, Jeffrey J.
包括形态发生、组织修复和肿瘤转移在内的基本生物过程需要细胞的集体运动（1-3），为了驱动这些运动，细胞对周围环境施加牵引力(4)。目前的理解强调，这些牵引力主要产生于前进的细胞片前缘的“先导细胞”（5-9）。我们的数据与这一假设相反，并首次通过直接测量显示，驱动集体细胞迁移的牵引力主要出现在前沿后面的许多细胞行，并延伸到很远的距离。此外，牵引力波动是异常的，表现出以指数尾为特征的广泛非高斯分布（10-12）。综上所述，这些意想不到的发现表明，尽管领导细胞可能在局部细胞引导中起着关键作用，但它产生的物理力量只是全球拔河的一小部分，涉及远远离前沿的细胞。
Fundamental biological processes including morphogenesis, tissue repair and tumour metastasis require collective cell motions(1-3), and to drive these motions cells exert traction forces on their surroundings(4). Current understanding emphasizes that these traction forces arise mainly in 'leader cells' at the front edge of the advancing cell sheet(5-9). Our data are contrary to that assumption and show for the first time by direct measurement that traction forces driving collective cell migration arise predominately many cell rows behind the leading front edge and extend across enormous distances. Traction fluctuations are anomalous, moreover, exhibiting broad non-Gaussian distributions characterized by exponential tails(10-12). Taken together, these unexpected findings demonstrate that although the leader cell may have a pivotal role in local cell guidance, physical forces that it generates are but a small part of a global tug-of-war involving cells well back from the leading edge.