EFFECT OF AMINO-ACIDS ON THE PRODUCTION OF CYCLOSPORINE A BY TOLYPOCLADIUM-INFLATUM
EFFECT OF AMINO-ACIDS ON THE PRODUCTION OF CYCLOSPORINE A BY TOLYPOCLADIUM-INFLATUM
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DOI：
10.1007/bf01192178
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发表时间：
1989-02-01
影响因子：
2.7
通讯作者：
AGATHOS, SN
中科院分区：
文献类型：
--
作者：
LEE, J;AGATHOS, SN
Cyclosporin A was produced in semi-synthetic and synthetic media by Tolypocladium inflatum in suspension culture. L-valine increased the specific production of cyclosporin A by 62% in semi-synthetic media and by 4 times in synthetic media, compared to an unsupplemented control culture. D-valine had no stimulatory effect on the production. L-leucine was also a strong enhancer of drug production in synthetic media. Experiments with different times of addition of L-valine indicated that the amino acid may need to be present in exponential growth phase for optimal production. 4g/l was the optimum concentration of exogenous L-valine. In contrast, exogenous L-methionine and sarcosine tended to diminish the production of cyclosporin A.
