Receptor-mediated protein sorting to the vacuole in yeast: roles for a protein kinase, a lipid kinase and GTP-binding proteins.
Receptor-mediated protein sorting to the vacuole in yeast: roles for a protein kinase, a lipid kinase and GTP-binding proteins.
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DOI：
10.1146/annurev.cb.11.110195.000245
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发表时间：
1995
影响因子：
11.3
通讯作者：
J. Stack;B. Horazdovsky;S. Emr
中科院分区：
文献类型：
--
作者：
J. Stack;B. Horazdovsky;S. Emr
在这篇综述中，我们总结了VPS（vacuolar protein sorting）基因产物的结构和功能特征，这些基因产物为真核细胞中蛋白质分选途径的调控相互作用和分子机制提供了深入的了解。酵母中的遗传选择已经鉴定了40多个囊泡介导的蛋白质分选到溶酶体样空泡所需的基因。这些VPS基因产物的分子表征揭示了参与这一过程的一些生化活动。与哺乳动物细胞中的甘露糖-6-磷酸受体类似，VPS 10基因编码酵母液泡水解酶羧肽酶Y的跨膜分选受体。VPS 15和VPS 34基因编码的一种新的信号转导复合物的可溶性液泡水解酶的传递所必需的组件。VPS 15和VPS 34分别编码丝氨酸/苏氨酸蛋白激酶和磷脂酰肌醇3-激酶，它们在细胞内膜区室的细胞质面相互作用，最有可能对应于晚期高尔基体。其他VPS基因产物具有参与膜运输途径的同源物：VPS 1和VPS 21基因分别编码发动蛋白和rab家族的GTPases，VPS 33、VPS 45和PEP 12/VPS 6基因的产物是参与调节的蛋白质的同源物突触囊泡胞吐作用。VPS基因产物构成了负责识别、包装和将蛋白质囊泡运输到酵母液泡中的分子装置的组成部分。
In this review we summarize the structural and functional characteristics of the VPS (vacuolar protein sorting) gene products that have provided insight into the regulatory interactions and molecular mechanisms underlying protein sorting pathways in eukaryotic cells. Genetic selections in yeast have resulted in the identification of more than 40 genes required for the vesicle-mediated sorting of proteins to the lysosome-like vacuole. Molecular characterization of these VPS gene products has revealed a number of biochemical activities involved in this process. Analogous to the mannose-6-phosphate receptors in mammalian cells, the VPS10 gene encodes a transmembrane sorting receptor for the yeast vacuolar hydrolase carboxypeptidase Y. The VPS15 and VPS34 genes encode components of a novel signal transduction complex essential for the delivery of soluble vacuolar hydrolases. VPS15 and VPS34 encode a serine/ threonine protein kinase and a phosphatidylinositol 3-kinase, respectively, that interact at the cytoplasmic face of an intracellular membrane compartment, most likely corresponding to the late Golgi. Other VPS gene products have homologues that are involved in membrane trafficking pathways: The VPSI and VPS21 genes encode GTPases of the dynamin and rab families, respectively, and the products of the VPS33, VPS45, and PEP12/VPS6 genes are homologues of proteins involved in regulated synaptic vesicle exocytosis. The VPS gene products constitute components of a molecular apparatus responsible for the recognition, packaging, and vesicular transport of proteins to the vacuole in yeast.